Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Lenny M. Seligman"'
Publikováno v:
New Directions for Institutional Research. 2017:25-36
This chapter demonstrates the complexity of pedagogical and curricular change as it unfolds through several overlapping phases of increasingly coordinated reflection and action around STEM initiatives at Pomona College. It argues for a networked mode
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::45c6cf7c4af4c5e03db22e9796c6bf36
https://doi.org/10.1002/ir.20210
https://doi.org/10.1002/ir.20210
Autor:
Jeremiah Savage, Meggen S. Chadsey, Adeline L. Veillet, Lenny M. Seligman, Karen M. Chisholm, Samuel T. Edwards, Brett Chevalier
Publikováno v:
Nucleic Acids Research. 30:3870-3879
The homing endonuclease I-CreI recognizes and cleaves a particular 22 bp DNA sequence. The crystal structure of I-CreI bound to homing site DNA has previously been determined, leading to a number of predictions about specific protein-DNA contacts. We
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45dec7dd220e7cbe1a42042d78afc593
https://doi.org/10.1093/nar/gkf495
https://doi.org/10.1093/nar/gkf495
Publikováno v:
Genetics. 147:1653-1664
We have developed and used a genetic selection system in Escherichia coli to study functional requirements for homing site recognition and cleavage by a representative eukaryotic mobile intron endonuclease. The homing endonuclease, I-CreI, was origin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::203805cbe216eb791359fc26742749f6
https://doi.org/10.1093/genetics/147.4.1653
https://doi.org/10.1093/genetics/147.4.1653
Publikováno v:
Journal of Bacteriology. 177:2315-2320
The Escherichia coli serine chemoreceptor (Tsr) is a protein with a simple topology consisting of two membrane-spanning sequences (TM1 and TM2) separating a large periplasmic domain from N-terminal and C-terminal cytoplasmic regions. We analyzed the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::008d4dc798db4db53f07a947120e7b4b
https://doi.org/10.1128/jb.177.9.2315-2320.1995
https://doi.org/10.1128/jb.177.9.2315-2320.1995
Autor:
Lenny M. Seligman, Colin Manoil
Publikováno v:
Journal of Biological Chemistry. 269:19888-19896
We developed a screen involving alkaline phosphatase gene fusions to identify mutations altering the membrane topology of a bacterial chemoreceptor (Escherichia coli Tsr). We identified three informative classes of mutations causing increased export
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dcdc0372f400fbbfe8a36685881053f2
https://doi.org/10.1016/s0021-9258(17)32103-8
https://doi.org/10.1016/s0021-9258(17)32103-8
Autor:
Brendan Springstubb, Barry L. Stoddard, Selma Masri, Django Sussman, Holly A. Morrison, Laura E. Rosen, Michael J. Brown, Lenny M. Seligman
Publikováno v:
Nucleic Acids Research
Homing endonucleases are highly specific enzymes, capable of recognizing and cleaving unique DNA sequences in complex genomes. Since such DNA cleavage events can result in targeted allele-inactivation and/or allele-replacement in vivo, the ability to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91ab76c07184e5481d6f214a66b69299
https://pubmed.ncbi.nlm.nih.gov/16971456
https://pubmed.ncbi.nlm.nih.gov/16971456
Autor:
Lenny M. Seligman, Alex Engel, Meg Chadsey, Barry L. Stoddard, Anna Bruett, Raymond J. Monnat, Steve Fauce, Django Sussman
Publikováno v:
Journal of molecular biology. 342(1)
Homing endonucleases are highly specific DNA endonucleases, encoded within mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites. Due to their biological function and hig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abc5c4437e423d330d81888375ad1c1b
https://pubmed.ncbi.nlm.nih.gov/15313605
https://pubmed.ncbi.nlm.nih.gov/15313605