Generation of sheep with defined FecB^B and TBXT mutations and porcine blastocysts with KCNJ5^G151R/+ mutation using prime editing.

Autor: Zhou, Shiwei^1,2 (AUTHOR), Lenk, Laura Johanna³ (AUTHOR), Gao, Yawei² (AUTHOR), Wang, Yuhui² (AUTHOR), Zhao, Xiaoe¹ (AUTHOR), Pan, Menghao¹ (AUTHOR), Huang, Shuhong² (AUTHOR), Sun, Kexin² (AUTHOR), Kalds, Peter^2,4 (AUTHOR), Luo, Qi² (AUTHOR), Lillico, Simon⁵ (AUTHOR), Sonstegard, Tad⁶ (AUTHOR), Scholl, Ute I.⁷ (AUTHOR), Ma, Baohua¹ (AUTHOR), Petersen, Bjoern³ (AUTHOR) bjoern.petersen@fli.de, Chen, Yulin^2,8 (AUTHOR) chenyulin@nwafu.edu.cn, Wang, Xiaolong^2,8 (AUTHOR) xiaolongwang@nwafu.edu.cn

Publikováno v: BMC Genomics. 6/12/2023, Vol. 24 Issue 1, p1-9. 9p.

Additional file 2 of Generation of sheep with defined FecBB and TBXT mutations and porcine blastocysts with KCNJ5G151R/+ mutation using prime editing

Autor: Zhou, Shiwei, Lenk, Laura Johanna, Gao, Yawei, Wang, Yuhui, Zhao, Xiaoe, Pan, Menghao, Huang, Shuhong, Sun, Kexin, Kalds, Peter, Luo, Qi, Lillico, Simon, Sonstegard, Tad, Scholl, Ute I., Ma, Baohua, Petersen, Bjoern, Chen, Yulin, Wang, Xiaolong

Additional file 2: Table S1. List of primers used for genotyping and amplifying PE3-targeted FecBB fragment in HEK293T cells. Table S2. Sequences of pegRNA and sgRNA used in human HEK293T cells. Table S3. Sequences of pegRNAs and sgRNAs used in sheep

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