Zobrazeno 1 - 10
of 29
pro vyhledávání: '"Lena Thoring"'
Autor:
Jeffrey L. Schloßhauer, Anne Zemella, Srujan K. Dondapati, Lena Thoring, Manpreet Meyer, Stefan Kubick
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-11 (2023)
Abstract Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution. Indeed, various functional groups including bul
Externí odkaz:
https://doaj.org/article/9837d159f19f4fed8152db056c167e33
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
The investigation of protein structures, functions and interactions often requires modifications to adapt protein properties to the specific application. Among many possible methods to equip proteins with new chemical groups, the utilization of ortho
Externí odkaz:
https://doaj.org/article/5fe100a93c9b4472bdf838299d44ceb2
Autor:
Srujan Kumar Dondapati, Henning Lübberding, Anne Zemella, Lena Thoring, Doreen A. Wüstenhagen, Stefan Kubick
Publikováno v:
Frontiers in Pharmacology, Vol 10 (2019)
Cell-free protein synthesis (CFPS) based on eukaryotic Sf21 lysate is gaining interest among researchers due to its ability to handle the synthesis of complex human membrane proteins (MPs). Additionally Sf21 cell-free systems contain endogenous micro
Externí odkaz:
https://doaj.org/article/39ec09c8196144baa1d5b3d3ba3ae5d1
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0215897 (2019)
The Toll-like receptor family belongs to the group of pathogen recognition receptors which is responsible for the discrimination of self and non-self pathogen-associated molecular patterns (PAMP's). Toll-like receptors play an important role in the i
Externí odkaz:
https://doaj.org/article/de3f9feb9b074b1b910a3383494dc8e6
Publikováno v:
Methods and Protocols, Vol 2, Iss 2, p 30 (2019)
In the biopharmaceutical pipeline, protein expression systems are of high importance not only for the production of biotherapeutics but also for the discovery of novel drugs. The vast majority of drug targets are proteins, which need to be characteri
Externí odkaz:
https://doaj.org/article/18e5bbd211604b3aab23627b64f6fe13
Autor:
Lena Thoring, Doreen A Wüstenhagen, Maria Borowiak, Marlitt Stech, Andrei Sonnabend, Stefan Kubick
Publikováno v:
PLoS ONE, Vol 11, Iss 9, p e0163670 (2016)
Nowadays, biotechnological processes play a pivotal role in target protein production. In this context, Chinese Hamster Ovary (CHO) cells are one of the most prominent cell lines for the expression of recombinant proteins and revealed as a safe host
Externí odkaz:
https://doaj.org/article/1b05bfc4ebda4570b27df2b98e320a15
Publikováno v:
Frontiers in molecular biosciences. 9
The investigation of protein structures, functions and interactions often requires modifications to adapt protein properties to the specific application. Among many possible methods to equip proteins with new chemical groups, the utilization of ortho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11a3fb1907cd244359edbded9fde27fd
https://pubmed.ncbi.nlm.nih.gov/35586195
https://pubmed.ncbi.nlm.nih.gov/35586195
Autor:
Marlitt, Stech, Nathanaël, Rakotoarinoro, Tamara, Teichmann, Anne, Zemella, Lena, Thoring, Stefan, Kubick
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2305
Cell-free protein synthesis (CFPS) enables the development of antibody conjugates, such as fluorophore conjugates and antibody-drug conjugates (ADCs), in a rapid and straightforward manner. In the first part, we describe the cell-free synthesis of an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c30c7c015c2e8ba7e1a93234b600882d
https://pubmed.ncbi.nlm.nih.gov/33950390
https://pubmed.ncbi.nlm.nih.gov/33950390
Autor:
Lena Thoring, Anne Zemella, Tamara Teichmann, Nathanaël Rakotoarinoro, Marlitt Stech, Stefan Kubick
Publikováno v:
Methods in Molecular Biology ISBN: 9781071614051
Cell-free protein synthesis (CFPS) enables the development of antibody conjugates, such as fluorophore conjugates and antibody-drug conjugates (ADCs), in a rapid and straightforward manner. In the first part, we describe the cell-free synthesis of an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9f08f6d18e21bb340bb47157f82b128b
https://doi.org/10.1007/978-1-0716-1406-8_9
https://doi.org/10.1007/978-1-0716-1406-8_9
This is part 3.3 of the "A Combined Cell-Free Protein Synthesis and Fluorescence-Based Approach to Investigate GPCR Binding Properties" collection of protocols: https://www.protocols.io/view/a-combined-cell-free-protein-synthesis-and-fluores-bqntmven
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::13314557cd4129d9602bb3cddf259607
https://doi.org/10.17504/protocols.io.bqnwmvfe
https://doi.org/10.17504/protocols.io.bqnwmvfe