Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Lena, Hörnsten"'
Publikováno v:
European Journal of Biochemistry. 269:2690-2697
Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f63a62612e28e7194de8dbf0acbd8e4e
https://doi.org/10.1046/j.1432-1033.2002.02936.x
https://doi.org/10.1046/j.1432-1033.2002.02936.x
Autor:
Lena Hörnsten, Ulf Hellman, Anne Osbourn, Paola Garosi, Chao Su, Ernst H. Oliw, Christer Wernstedt
Publikováno v:
Journal of Biological Chemistry. 274:28219-28224
Linoleate diol synthase is a homotetrameric ferric hemeprotein, which catalyzes dioxygenation of linoleic acid to (8R)-hydroperoxylinoleate and isomerization of the hydroperoxide to (7S,8S)-dihydroxylinoleate. Ferryl intermediates and a tyrosyl radic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e074a04240b2bca99c1a00e56bd4dc36
https://doi.org/10.1074/jbc.274.40.28219
https://doi.org/10.1074/jbc.274.40.28219
Autor:
Helena Lindgren, Helena Hedlund, Therese Graner, Pierre Le Greves, Lars Andersson, Staffan Lindqvist, Mattias Algotsson, Katarina Öberg, Lena Hörnsten, Marianne Carlsson, Maria Björner, Gunnar Glad
Immobilized metal affinity chromatography (IMAC) purification of secreted histidine-tagged proteins in eukaryotic cell culture supernatants is often problematic. Incompatibility with the cell culture media appears as stripping of the immobilized meta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5624594cbc92695b82b69243c429092
https://europepmc.org/articles/PMC3635365/
https://europepmc.org/articles/PMC3635365/
Publikováno v:
Archives of Biochemistry and Biophysics. 305:288-297
Incubation of 5,8,11-[1- 14 C]eicosatrienoic acid with prostaglandin endoperoxide synthase of ram vesicular gland microsomes led to formation of a number of polar metabolites. Four major compounds were characterized by chemical and physical methods a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e14104efbfbb10137bc55079a2ed543
https://doi.org/10.1006/abbi.1993.1425
https://doi.org/10.1006/abbi.1993.1425
Publikováno v:
Archives of Biochemistry and Biophysics. 300:434-439
[ 14 C]Linoleic acid was incubated with phenobarbital-induced rat liver microsomes and formation of cis-trans -conjugated hydroxy fatty acids was investigated. 13-Hydroxy-9 Z ,11 E -octadecadienoic acid (13-HODE), 9-hydroxy-10 E ,12 Z -octadecadienoi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::765c65820c8b5116233e060b8b6bbebc
https://doi.org/10.1006/abbi.1993.1059
https://doi.org/10.1006/abbi.1993.1059
Publikováno v:
Archives of biochemistry and biophysics. 434(1)
Manganese lipoxygenase is secreted by the fungus Gaeumannomyces graminis. We expressed the enzyme in Pichia pastoris, which secreted approximately 30 mg Mn-lipoxygenase/L culture medium in fermentor. The recombinant lipoxygenase was N- and O-glycosyl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ace728d143fefdead9abf8f33cbf7b27
https://pubmed.ncbi.nlm.nih.gov/15629124
https://pubmed.ncbi.nlm.nih.gov/15629124
Autor:
Jean-Luc Maloisel, Mari-Ann Pesula, Ronnie Palmgren, Camilla Larsson, Maria Liminga, Rama Bhikhabhai, Ulrika Carlsson, Mattias Algotsson, John Flensburg, Lena Hörnsten
Publikováno v:
Methods in Proteome and Protein Analysis ISBN: 9783642057793
Knowledge of protein and peptide sequences is fundamentally important for understanding many physiological and biochemical processes at the molecular level. Chemically assisted fragmentation by MALDI (matrix-assisted laser desorption/ionization mass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89eb04dd16d8769cce20c1be7e2ecec7
https://doi.org/10.1007/978-3-662-08722-0_19
https://doi.org/10.1007/978-3-662-08722-0_19
Publikováno v:
Advances in experimental medicine and biology. 507
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f9e11aa9aae4fadbc751304cbde7bcc5
https://pubmed.ncbi.nlm.nih.gov/12664640
https://pubmed.ncbi.nlm.nih.gov/12664640
Publikováno v:
European journal of biochemistry. 269(11)
Manganese lipoxygenase was isolated to homogeneity from the take-all fungus, Gaeumannomyces graminis. The C-terminal amino acids and several internal peptides were sequenced, and the information was used to obtain a cDNA probe by RT/PCR. Screening of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::90bf32a8b5237dbdf66c027915eec91e
https://pubmed.ncbi.nlm.nih.gov/12047377
https://pubmed.ncbi.nlm.nih.gov/12047377
Publikováno v:
Advances in Experimental Medicine and Biology ISBN: 9781461349600
Dioxygenation of polyunsaturated fatty acids is catalyzed by lipoxygenases, prostaglandin H (PGH) synthases, and linoleate diol synthase (1-8). Lipoxygenases are non-heme proteins with a mononuclear metal center with either iron or manganese (1,8-9).
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ff459459f0d7020f21e9b5466d9e082
https://doi.org/10.1007/978-1-4615-0193-0_85
https://doi.org/10.1007/978-1-4615-0193-0_85