Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Len Pagliaro"'
Publikováno v:
SLAS Discovery. 8:566-570
Several factors are known to increase the noise and variability of cell-based assays used for high-throughput screening. In particular, edge effects can result in an unacceptably high plate rejection rate in screening runs. In an effort to minimize t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::68c9af4492fb10482f6f60609068890e
https://doi.org/10.1177/1087057103256465
https://doi.org/10.1177/1087057103256465
Publikováno v:
Journal of Nanomaterials, Vol 2014 (2014)
Photothermal therapy (PTT) treatments have shown strong potential in treating tumors through their ability to target destructive heat preferentially to tumor regions. In this paper we demonstrate that PTT in a murine melanoma model using gold nanorod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6aa3abcb6910f852b3c81327cc629829
https://doaj.org/article/323ce4131e154052b3d3ba12b1642439
https://doaj.org/article/323ce4131e154052b3d3ba12b1642439
Autor:
Morten Præstegaard, Len Pagliaro
Publikováno v:
SLAS Discovery. 6:133-136
During 1999, Journal of Biomolecular Screening presented a series of Point-Counterpoint articles that addressed a question posed by editor Bill Janzen: "What is the future of HTS?" These articles discussed many of the global issues involved in HTS, s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5c9adda5c9e461d80d5a68ab55a8ca4
https://doi.org/10.1177/108705710100600302
https://doi.org/10.1177/108705710100600302
The Molecular Nature of the F-actin Binding Activity of Aldolase Revealed with Site-directed Mutants
Publikováno v:
Journal of Biological Chemistry. 271:6861-6865
We used site-directed mutagenesis of rabbit muscle aldolase, falling ball viscometry, co-sedimentation binding assays, and negative stain electron microscopy, to identify specific residues involved in the aldolase-actin interaction. Three mutants, R4
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a9f16ad64c6adf202d600385ec805690
https://doi.org/10.1074/jbc.271.12.6861
https://doi.org/10.1074/jbc.271.12.6861
Publikováno v:
Methods in enzymology. 414
Redistribution technology is a cell-based assay technology that uses protein translocation as the primary readout for the activity of cellular signaling pathways and other intracellular events. Protein targets are labeled with the green fluorescent p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2e56439c9043a078514a902fb2ae2c4b
https://pubmed.ncbi.nlm.nih.gov/17110209
https://pubmed.ncbi.nlm.nih.gov/17110209
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 356
This chapter describes the design and development of cell-based assays, in which quantitation of the intracellular translocation of a target protein--rather than binding or catalytic activity--provides the primary assay readout. These are inherently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::16c83ad33de9c1b9fee9721a4b5cd073
https://pubmed.ncbi.nlm.nih.gov/16988419
https://pubmed.ncbi.nlm.nih.gov/16988419
This chapter describes the design and development of cell-based assays, in which quantitation of the intracellular translocation of a target protein--rather than binding or catalytic activity--provides the primary assay readout. These are inherently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f87e54ad19efe338dce871cda5f3c11
https://doi.org/10.1385/1-59745-217-3:401
https://doi.org/10.1385/1-59745-217-3:401
Redistribution technology is a cell-based assay technology that uses protein translocation as the primary readout for the activity of cellular signaling pathways and other intracellular events. Protein targets are labeled with the green fluorescent p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b9efe8ae4d5353552ba6e46532543dc9
https://doi.org/10.1016/s0076-6879(06)14027-6
https://doi.org/10.1016/s0076-6879(06)14027-6
Autor:
Robert Terry, Dorthe Christensen Almholt, Soren Weis Dahl, Steven Butcher, Christian Krog-Jensen, Søren Møller, Sara Petersen Bjørn, Anthony J. Mason, Morten Praestegaard, Frosty Loechel, Morten Heide, Len Pagliaro, Søren Jensby Nielsen, Pedersen Hans Christian
Publikováno v:
Assay and drug development technologies. 2(1)
Redistribution (BioImage) A/S, Soborg, Denmark) is a novel high-throughput screening technology that monitors translocation of specific protein components of intracellular signaling pathways within intact mammalian cells, using green fluorescent prot
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c6a2caba04b66f27441608eb6b14f792
https://pubmed.ncbi.nlm.nih.gov/15090206
https://pubmed.ncbi.nlm.nih.gov/15090206
Publikováno v:
Journal of biomolecular screening. 8(5)
Several factors are known to increase the noise and variability of cell-based assays used for high-throughput screening. In particular, edge effects can result in an unacceptably high plate rejection rate in screening runs. In an effort to minimize t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::15ee4035e26185c412e4cd4ff1cca582
https://pubmed.ncbi.nlm.nih.gov/14567784
https://pubmed.ncbi.nlm.nih.gov/14567784