Zobrazeno 1 - 10
of 41
pro vyhledávání: '"Leifu Chang"'
Autor:
Clinton A. Gabel, Zhuang Li, Andrew G. DeMarco, Ziguo Zhang, Jing Yang, Mark C. Hall, David Barford, Leifu Chang
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)
The eight-subunit augmin complex is required to nucleate branching microtubules and create a robust mitotic spindle during cell division. Here, the authors use cryo-EM, crosslinking mass spectrometry, and computational tools to build a structural mod
Externí odkaz:
https://doaj.org/article/21dc5e7397464617b4ce405536bc7e4b
Autor:
Leifu Chang, Jing Yang, Chang Hwa Jo, Andreas Boland, Ziguo Zhang, Stephen H. McLaughlin, Afnan Abu-Thuraia, Ryan C. Killoran, Matthew J. Smith, Jean-Francois Côté, David Barford
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
DOCK2 is a guanine nucleotide exchange factor (GEF) that activates RHO GTPases and interacts with ELMO1, which stimulates its GEF activity. Here, the authors provide mechanistic insights into how ELMO1 regulates DOCK2 activity by determining the stru
Externí odkaz:
https://doaj.org/article/d8a372772f44414b8c28c69fb426524f
Publikováno v:
Nat Chem Biol
Anti-CRISPR (Acr) proteins are encoded by phages to inactivate CRISPR-Cas systems of bacteria and archaea and are used to enhance the CRISPR toolbox for genome editing. Here we report the structure and mechanism of AcrIF24, an Acr protein that inhibi
Autor:
Zhuang Li, Anthony A. Kossiakoff, Qiuyan Chen, Xin-Qiu Yao, Leifu Chang, Thomas Klose, Somnath Mukherjee, Chun-Liang Chen, Manolo Plasencia, Dhabaleswar Patra, Philip C. Andrews, John J.G. Tesmer
Publikováno v:
Nature
G-protein-coupled receptor (GPCR) kinases (GRKs) selectively phosphorylate activated GPCRs, thereby priming them for desensitization1. Although it is unclear how GRKs recognize these receptors2-4, a conserved region at the GRK N terminus is essential
Publikováno v:
Nucleic Acids Research
Cas12f, also known as Cas14, is an exceptionally small type V-F CRISPR–Cas nuclease that is roughly half the size of comparable nucleases of this type. To reveal the mechanisms underlying substrate recognition and cleavage, we determined the cryo-E
Publikováno v:
Nat Chem Biol
Cas12g, the type V–G CRISPR–Cas effector, is an RNA-guided ribonuclease that targets single-stranded RNA substrate. The CRISPR–Cas12g system offers a potential platform for transcriptome engineering and diagnostic applications. We determined th
Publikováno v:
Nucleic Acids Research
CRISPR–Cas systems are adaptive immune systems in bacteria and archaea to defend against mobile genetic elements (MGEs) and have been repurposed as genome editing tools. Anti-CRISPR (Acr) proteins are produced by MGEs to counteract CRISPR–Cas sys
Autor:
David Barford, Chang Hwa Jo, Matthew J. Smith, Ziguo Zhang, Jean-François Côté, Stephen H. McLaughlin, Jing Yang, Andreas Boland, Afnan Abu-Thuraia, Leifu Chang, Ryan C. Killoran
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-17 (2020)
Nature Communications
Nature Communications
Funder: Gouvernement du Canada | Instituts de Recherche en Santé du Canada | CIHR Skin Research Training Centre (Skin Research Training Centre); doi: https://doi.org/10.13039/501100007202
Funder: Canadian Network for Research and Innovation in
Funder: Canadian Network for Research and Innovation in
Autor:
Shukun Wang, Zhuang Li, Ruijie Han, Indranil Mukherjee, Clinton Gabel, Leifu Chang, Renjian Xiao
Publikováno v:
Mol Cell
The type V-K CRISPR-Cas system, featured by Cas12k effector with a naturally inactivated RuvC domain and associated with Tn7-like transposon for RNA-guided DNA transposition, is a promising tool for precise DNA insertion. To reveal the mechanism unde
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a6e1dd0b67550ac2699169884ca1132
https://doi.org/10.1101/2021.07.07.451486
https://doi.org/10.1101/2021.07.07.451486
Autor:
Thomas Klose, Manolo Plasencia, Philip C. Andrews, John J.G. Tesmer, Xin-Qiu Yao, Zhuang Li, Dhableswar Patra, Chun-Liang Chen, Qiuyan Chen, Leifu Chang, Anthony A. Kossiakoff, Somnath Mukherjee
Publikováno v:
The FASEB Journal. 35