Zobrazeno 1 - 10
of 82
pro vyhledávání: '"Leif A. Isaksson"'
Publikováno v:
FEBS Journal. 278:3508-3517
A mutation in the infA gene encoding initiation factor 1 (IF1) gives rise to a cold-sensitive phenotype. An Escherichia coli strain with this mutation was used as a tool to select for second-site suppressors that compensate for the cold sensitivity a
Publikováno v:
FEBS Journal. 278:1745-1756
Genetic selection has been used to isolate second-site suppressors of a defective cold-sensitive initiation factor I (IF1) R69L mutant of Escherichia coli. The suppressor mutants specifically map t ...
Autor:
Louise Carøe Vohlander Rasmussen, Hanna Nilsson, Serhiy Surkov, Leif A. Isaksson, Hans Uffe Sperling-Petersen
Publikováno v:
FEBS Journal. 277:2428-2439
Translation initiation factor 1 (IF1) is an essential protein in prokaryotes. The nature of IF1 interactions with the mRNA during translation initiation on the ribosome remains unclear, even though ...
Publikováno v:
Journal of Bacteriology. 189:8993-9000
Expression of minigenes encoding tetra- or pentapeptides MXLX or MXLXV (E peptides), where X is a nonpolar amino acid, renders cells erythromycin resistant whereas expression of minigenes encoding tripeptide MXL does not. By using a 3A′ reporter ge
Publikováno v:
Microbial Cell Factories
Background Arginine is a high-value product, especially for the pharmaceutical industry. Growing demand for environmental-friendly and traceable products have stressed the need for microbial production of this amino acid. Therefore, the aim of this s
Autor:
Alhad A. Mahagaonkar, Leif A. Isaksson, Elizabeth S. Poole, Louise L. Major, Warren P. Tate, Andrew G. Cridge
Publikováno v:
Nucleic Acids Research
Six diverse prokaryotic and five eukaryotic genomes were compared to deduce whether the protein synthesis termination signal has common determinants within and across both kingdoms. Four of the six prokaryotic and all of the eukaryotic genomes invest
Publikováno v:
FEBS Letters. 579:995-1000
The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A′ reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutant
Publikováno v:
Experimental Cell Research. 300:190-201
Electron tomography (ET) has been used to reconstruct in situ individual 50S ribosomal subunits in Escherichia coli rifampicin-treated cells. Rifampicin inhibits transcription initiation. As a result, rapid degradation of preformed mRNA and dissociat
Publikováno v:
Journal of Biotechnology. 111:17-30
Uneven distribution of plasmid-based expression vectors to daughter cells during bacterial cell division results in an increasing proportion of plasmid free cells during growth. This is a major industrial problem leading to reduction of product yield
Autor:
Victor Croitoru, Leif A. Isaksson, Margarete Bucheli-Witschel, Farhad Maruf Abdulkarim, Peter Jorgen Hagg
Publikováno v:
European Journal of Biochemistry. 271:534-544
Three protein factors IF1, IF2 and IF3 are involved in the initiation of translation in prokaryotes. No clear function has been assigned to the smallest of these three factors, IF1. Therefore, to investigate the role of this protein in the initiation