Low-dose cryo-electron ptychography of proteins at sub-nanometer resolution.

Autor: Küçükoğlu B; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland., Mohammed I; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland., Guerrero-Ferreira RC; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland.; Robert P. Apkarian Integrated Electron Microscopy Core, Emory University School of Medicine, 1521 Dickey Drive NE, Atlanta, GA, 30322, USA., Ribet SM; National Center for Electron Microscopy (NCEM), Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Varnavides G; National Center for Electron Microscopy (NCEM), Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Miller Institute for Basic Research in Science, University of California, Berkeley, CA, 94720, USA., Leidl ML; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany.; Department of Chemistry and Centre for NanoScience, Ludwig-Maximilians-Universität München, Butenandstr. 11, 81377, München, Germany., Lau K; Protein Production and Structure Core Facility (PTPSP), School of Life Sciences, EPFL, Rte Cantonale, 1015, Lausanne, Switzerland., Nazarov S; Dubochet Center for Imaging Lausanne, EPFL and UNIL, EPFL VPA DCI-Lausanne, 1015, Lausanne, Switzerland., Myasnikov A; Dubochet Center for Imaging Lausanne, EPFL and UNIL, EPFL VPA DCI-Lausanne, 1015, Lausanne, Switzerland., Kube M; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland., Radecke J; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland., Sachse C; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany.; Department of Biology, Heinrich Heine University, Düsseldorf, Germany., Müller-Caspary K; Department of Chemistry and Centre for NanoScience, Ludwig-Maximilians-Universität München, Butenandstr. 11, 81377, München, Germany., Ophus C; National Center for Electron Microscopy (NCEM), Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Stahlberg H; Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland. henning.stahlberg@epfl.ch.

Publikováno v: Nature communications [Nat Commun] 2024 Sep 14; Vol. 15 (1), pp. 8062. Date of Electronic Publication: 2024 Sep 14.

Single-particle cryo-EM structures from iDPC-STEM at near-atomic resolution.

Autor: Lazić I; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands. ivan.lazic@thermofisher.com., Wirix M; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands., Leidl ML; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany.; Department of Chemistry and Centre for NanoScience, Ludwig-Maximilians-University Munich, Munich, Germany.; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-1): Physics of Nanoscale Systems, Jülich, Germany.; Institute for Biological Information Processing (IBI-6): Cellular Structural Biology, Jülich, Germany., de Haas F; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands., Mann D; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany.; Institute for Biological Information Processing (IBI-6): Cellular Structural Biology, Jülich, Germany., Beckers M; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany.; Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany., Pechnikova EV; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands., Müller-Caspary K; Department of Chemistry and Centre for NanoScience, Ludwig-Maximilians-University Munich, Munich, Germany.; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-1): Physics of Nanoscale Systems, Jülich, Germany., Egoavil R; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands., Bosch EGT; Materials and Structural Analysis Division, Thermo Fisher Scientific, Eindhoven, Netherlands., Sachse C; Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons (ER-C-3): Structural Biology, Jülich, Germany. c.sachse@fz-juelich.de.; Institute for Biological Information Processing (IBI-6): Cellular Structural Biology, Jülich, Germany. c.sachse@fz-juelich.de.; Department of Biology, Heinrich Heine University, Düsseldorf, Germany. c.sachse@fz-juelich.de.

Publikováno v: Nature methods [Nat Methods] 2022 Sep; Vol. 19 (9), pp. 1126-1136. Date of Electronic Publication: 2022 Sep 05.