Zobrazeno 1 - 10
of 113
pro vyhledávání: '"Leevi Kääriäinen"'
Publikováno v:
Journal of Virology
Like other positive-strand RNA viruses, alphaviruses replicate their genomes in association with modified intracellular membranes. Alphavirus replication sites consist of numerous bulb-shaped membrane invaginations (spherules), which contain the doub
Autor:
Pertti Aula, Leevi Kääriäinen
Publikováno v:
Hereditas. 51:274-278
Publikováno v:
Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology. :760-768
By means of carbohydrate labelling two virus-specific glycoproteins were revealed in BHK21 cells infected with Semliki Forest virus: a non-structural protein (NSP68) and a structural protein (VP53). In addition, a third virus-induced, carbohydrate-co
Autor:
Leevi Kääriäinen, Hans Söderlund
Publikováno v:
Acta Pathologica Microbiologica Scandinavica Section B Microbiology and Immunology. :33-40
Semliki Forest virus specific polysomes from infected HeLa cells were isolated. Part of the newly formed, radioactively labelled protein remained associated with the messenger RNA after release of the nascent polypeptide chains with EDTA: The messeng
Publikováno v:
Journal of Virology
We have analyzed the biochemical consequences of mutations that affect viral RNA synthesis in Semliki Forest virus temperature-sensitive (ts) mutants. Of the six mutations mapping in the multifunctional replicase protein nsP2, three were located in t
Publikováno v:
Journal of Virology. 81:872-883
Semliki Forest virus RNA replication takes place in association with specific cytoplasmic vacuoles, derived from the endosomal apparatus. Of the four virus-encoded replicase proteins, nsP1 serves as the membrane anchor of the replication complex. An
Publikováno v:
European Journal of Biochemistry. 112:461-468
A large short-lived virus-specific nonstructural protein with an apparent molecular weight of about 250000 (nsp250) has been isolated from cells infected with the temperature-sensitive mutants ts-4 and ts-6 of the Semliki Forest virus. nsp250 contain
Publikováno v:
Applied Microbiology and Biotechnology
The search for inhibitors of viral replication is dependent on understanding the events taking place at the molecular level during viral infection. All the essential steps during the viral life cycle are potential targets for antiviral drugs. Classic
Publikováno v:
Journal of Virology. 77:1691-1702
The late RNA synthesis in alphavirus-infected cells, generating plus-strand RNAs, takes place on cytoplasmic vacuoles (CPVs), which are modified endosomes and lysosomes. The cytosolic surface of CPVs consists of regular membrane invaginations or sphe
Publikováno v:
Journal of Virology
The nonstructural (ns) proteins nsP1 to -4, the components of Semliki Forest virus (SFV) RNA polymerase, were localized in infected cells by confocal microscopy using double labeling with specific antisera against the individual ns proteins. All ns p