Zobrazeno 1 - 10
of 232
pro vyhledávání: '"Leendert Bosch"'
Publikováno v:
European Journal of Biochemistry. 227:816-822
Pulvomycin is a strong inhibitor of protein synthesis, known to prevent the binding of aminoacyl-tRNA to elongation factor Tu · GTP (EF-Tu · GTP). Recently, three pulvomycin-resistant mutant strains have been isolated by targeted mutagenesis of the
Autor:
Barend Kraal, F. J. Duisterwinkel, Peter J. M. Schretlen, Leendert Bosch, J. Martiea De Graaf
Publikováno v:
European Journal of Biochemistry. 117:7-12
In the accompanying paper we have shown that polypeptide synthesis sustained by the mutant elongation factor EF-TuBO is inhibited by kirromycin. Here we have searched for the primary site of inhibition in the elongation cycle. It is demonstrated that
Autor:
A. H. Borman, Peter Westbroek, Marijke Gruter, Elisabeth W. de Vrind-de Jong, Leendert Bosch, Remi Thierry, Johannes P. Kamerling
Publikováno v:
Journal of Phycology. 23:118-123
Coccoliths of Emiliania huxleyi (Lohmann) Hay and Mohler, a unicellular calcifying alga, consist of calcite closely associated with an acidic, Ca2+-binding polysaccharide. This polysaccharide is thought to play a regulatory role in coccolith synthesi
Publikováno v:
Biochemical and Biophysical Research Communications. 225:961-967
We have developed a cell-free system of E. coli that enables us to study the in vitro translation of natural mRNA mediated by wild-type or mutant EF-Tu. Various mutant EF-Tu species have been analyzed, one of which, EF-Tu [G222D], appeared to be virt
Autor:
Kathy Boon, Barend Kraal, Andrea Parmeggiani, Pieter H. Anborgh, Leendert Bosch, Rolf Hilgenfeld, Leo A. H. Zeef, Jeroen R. Mesters, Erik L.H. Vorstenbosch
Publikováno v:
Biochemistry and Cell Biology. 73:1167-1177
Analysis of antibiotic-resistant EF-Tu mutants has revealed a connection between resistance and structural elements that participate in the GTPase switching mechanism. Both random and site-directed mutagenesis methods have yielded sets of purified mu
Publikováno v:
FEMS Microbiology Letters. 99:85-88
The FIS protein (factor for inversion stimulation) is known to activate the transcription of rRNA and tRNA operons in Escherichia coli. In this report the growth behaviour of a fis bacterium was studied under steady-state conditions and in competitio
Publikováno v:
Journal of Molecular Biology. 223:221-232
Site-directed mutations were introduced in the connecting loops and one of the two stem regions of the RNA pseudoknot in the tRNA-like structure of turnip yellow mosaic virus RNA. The kinetic parameters of valylation for each mutated RNA were determi
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1129:64-72
Light scattering studies of RNA isolated from turnip yellow mosaic virus (TYMV) revealed a molar mass of 1.9.10(6) g mol-1, which is close to the value of 2.0.10(6) g mol-1 published for intact genomic TYMV RNA (2M RNA). However, gel electrophoresis
Publikováno v:
European Journal of Biochemistry. 201:303-324
The structurally and functionally best known RNA molecule is undoubtedly transfer RNA (tRNA). tRNA owes its name to the central role it performs in protein biosynthesis: it mediates the transfer of amino acids to the ribosome. In this process the ami
Publikováno v:
Biochimie, 73(7-8), 1089-1092. EDITIONS SCIENTIFIQUES ELSEVIER
The turnover of EF-Tu·GTP on poly-U programmed ribosomes was measured both in the presence and in the absence of N-acetylated Phe-tRNA Phe at the P-site. The reaction was uncoupled fromrotein synthesis by omitting Phe-tRNA Phe at the A-site. In this