Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Leena Ukil"'
Autor:
Roy Joseph, Yuriy L Orlov, Mikael Huss, Wenjie Sun, Say Li Kong, Leena Ukil, You Fu Pan, Guoliang Li, Michael Lim, Jane S Thomsen, Yijun Ruan, Neil D Clarke, Shyam Prabhakar, Edwin Cheung, Edison T Liu
Publikováno v:
Molecular Systems Biology, Vol 6, Iss 1, Pp n/a-n/a (2010)
A major question in transcription factor (TF) biology is why a TF binds to only a small fraction of motif eligible binding sites in the genome. Using the estrogen receptor‐α as a model system, we sought to explicitly define parameters that determi
Externí odkaz:
https://doaj.org/article/7bdfe7fe36ae4c3688f07e0338e42aab
Publikováno v:
Genetics. 196:177-195
The nuclear pore complex proteins SonA and SonB, the orthologs of mammalian RAE1 and NUP98, respectively, were identified in Aspergillus nidulans as cold-sensitive suppressors of a temperature-sensitive allele of the essential mitotic NIMA kinase (ni
Autor:
Aysha H. Osmani, Michael J. Hynes, Berl R. Oakley, Tania Nayak, Edyta Szewczyk, Leena Ukil, C. Elizabeth Oakley, Stephen A. Osmani, Sandra L. Murray
Publikováno v:
Genetics. 172:1557-1566
Aspergillus nidulans is an important experimental organism, and it is a model organism for the genus Aspergillus that includes serious pathogens as well as commercially important organisms. Gene targeting by homologous recombination during transforma
Autor:
Aysha H. Osmani, Jonathan Davies, Stephen A. Osmani, Leena Ukil, Ariadna Perez-Balaguer, Lin Yang, Colin P. De Souza, Francis Nahm, Xiaowei Dou
Publikováno v:
Eukaryotic Cell. 3:1359-1362
A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans . The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres throug
Autor:
Sarine Markossian, Archana Varadaraj, Shahr B. Hashmi, Kuo-Fang Shen, Leena Ukil, Colin P. De Souza, Sunghun Son, Stephen A. Osmani, Meera Govindaraghavan, Hui Lin Liu, Aysha H. Osmani
Publikováno v:
Eukaryotic Cell. 9:831-833
A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided.
Autor:
Valere Cacheux-Rataboul, Wing-Kin Sung, Per Hall, Edison T. Liu, Hao Zhao, Wan Ting Poh, Jonas Bergh, Kelson F B Zawack, Wah Heng Lee, Khay Guan Yeoh, Jimmy Bok Yan So, See Ting Leong, Xiaoan Ruan, Manuel Salto-Tellez, Guillaume Bourque, Yijun Ruan, Audrey S.M. Teo, Jieqi P. Chen, Zhenshui Zhang, Patrick Tan, Yee Yen Sia, Song Gao, Chia-Lin Wei, Chee Seng Chan, Pramila N. Ariyaratne, Feng Zhu, Vikrant Kumar, Xing Yi Woo, Koichiro Inaki, Fei Yao, Say Chuan Neo, Axel M. Hillmer, Guoliang Li, Poh Sum D Choi, Hui Ping J Lim, Hervé Thoreau, Leena Ukil, Niranjan Nagarajan, Atif Shahab
Publikováno v:
Genome research. 21(5)
Somatic genome rearrangements are thought to play important roles in cancer development. We optimized a long-span paired-end-tag (PET) sequencing approach using 10-Kb genomic DNA inserts to study human genome structural variations (SVs). The use of a
Autor:
Jane S. Thomsen, Neil D. Clarke, Yuriy L. Orlov, Michael Lim, Wenjie Sun, Leena Ukil, Say Li Kong, Yijun Ruan, Edwin Cheung, You Fu Pan, Mikael Huss, Shyam Prabhakar, Edison T. Liu, Guoliang Li, Roy Joseph
Publikováno v:
Molecular Systems Biology
Besides the estrogen receptor response elements (EREs), estrogen receptor-α binding is augmented by FOXA1 co-binding, the presence of the histone mark, histone 3 monomethylated at the lysine 4 position and the presence of open chromatin. The major d
Publikováno v:
Molecular biology of the cell. 20(8)
How the nucleolus is segregated during mitosis is poorly understood and occurs by very different mechanisms during closed and open mitosis. Here we report a new mechanism of nucleolar segregation involving removal of the nucleolar-organizing regions
Publikováno v:
Eukaryotic cell. 7(12)
The Aspergillus nidulans NIMA kinase is essential for mitosis and is the founding member of the conserved N IMA-r e lated k inase (Nek) family of protein kinases. To gain insight into NIMA function, a copy number suppression screen has been completed
Autor:
Weiling An, Dongliang Wu, Shahr B. Hashmi, Leena Ukil, Jonathan Davies, Stephen A. Osmani, Xiaowei Dou
Publikováno v:
Genetics. 165(3)
Unlike Pho85 of Saccharomyces cerevisiae, the highly related PHOA cyclin-dependent kinase (CDK) of Aspergillus nidulans plays no role in regulation of enzymes involved in phosphorous acquisition but instead modulates differentiation in response to en