Zobrazeno 1 - 10
of 134
pro vyhledávání: '"Lee D Spate"'
Publikováno v:
PLoS ONE, Vol 16, Iss 2, p e0243727 (2021)
In vitro embryo production systems are limited by their inability to consistently produce embryos with the competency to develop to the blastocyst stage, survive cryopreservation, and establish a pregnancy. Previous work identified a combination of t
Externí odkaz:
https://doaj.org/article/71da3c9206a6461e96368cda723d4032
Autor:
Lynda S Ostedgaard, Margaret P Price, Kristin M Whitworth, Mahmoud H Abou Alaiwa, Anthony J Fischer, Akshaya Warrier, Melissa Samuel, Lee D Spate, Patrick D Allen, Brieanna M Hilkin, Guillermo S Romano Ibarra, Miguel E Ortiz Bezara, Brian J Goodell, Steven E Mather, Linda S Powers, Mallory R Stroik, Nicholas D Gansemer, Camilla E Hippee, Keyan Zarei, J Adam Goeken, Thomas R Businga, Eric A Hoffman, David K Meyerholz, Randall S Prather, David A Stoltz, Michael J Welsh
Publikováno v:
eLife, Vol 9 (2020)
Submucosal glands (SMGs) are a prominent structure that lines human cartilaginous airways. Although it has been assumed that SMGs contribute to respiratory defense, that hypothesis has gone without a direct test. Therefore, we studied pigs, which hav
Externí odkaz:
https://doaj.org/article/de4e52b4b350483bb95240e3006ac61e
Autor:
Bethany K Redel, Benjamin P Beaton, Lee D Spate, Joshua A Benne, Stephanie L Murphy, Chad W O’Gorman, Anna M Spate, Randall S Prather, Kevin D Wells
Publikováno v:
BioTechniques, Vol 64, Iss 3, Pp 118-124 (2018)
Production of Cas9 mRNA in vitro typically requires the addition of a 5´ cap and 3´ polyadenylation. A plasmid was constructed that harbored the T7 promoter followed by the EMCV IRES and a Cas9 coding region. We hypothesized that the use of the met
Externí odkaz:
https://doaj.org/article/0c50dbd04e8e4a50b5173c1f244c4e26
Autor:
Lee D Spate, Alana N Brown, Bethany K Redel, Kristin M Whitworth, Clifton N Murphy, Randall S Prather
Publikováno v:
PLoS ONE, Vol 9, Iss 4, p e95114 (2014)
The ability to mature oocytes in vitro provides a tool for creating embryos by parthenogenesis, fertilization, and cloning. Unfortunately the quality of oocytes matured in vitro falls behind that of in vivo matured oocytes. To address this difference
Externí odkaz:
https://doaj.org/article/14bc68fa4ced4259b89000f63f0e43f7
Publikováno v:
PLoS ONE, Vol 8, Iss 6, p e65545 (2013)
Cryopreservation of swine embryos is inefficient. Our goal was to develop a non-invasive method for "relatively" high-throughput cryopreservation of in vivo-produced swine embryos. Since removal of the lipid droplets within early swine embryos improv
Externí odkaz:
https://doaj.org/article/28b25fd9c93b44e98b41bc5d5a016916
Autor:
Paula R Chen, Kyungjun Uh, Kaylynn Monarch, Lee D Spate, Emily D Reese, Randall S Prather, Kiho Lee
Publikováno v:
Biology of Reproduction. 108:611-618
Growth differentiation factor 9 (GDF9) is a secreted protein belonging to the transforming growth factor beta superfamily and has been well characterized for its role during folliculogenesis in the ovary. Although previous studies in mice and sheep h
Autor:
Melissa Samuel, Caroline A Pfeiffer, Destiny N Johns, Matthew C. Lucy, Kevin D. Wells, Melissa A Fudge, Lee D. Spate, Hongyu Liu, Shelbi D Perry, Caroline G Lucas, Paula R Chen, Christine Spinka, Rodney D. Geisert, Thomas E. Spencer, Raissa F. Cecil, Randall S. Prather, Ashley E Meyer
Publikováno v:
Biology of Reproduction. 105:1577-1590
Establishment and maintenance of pregnancy in the pig is a complex process that relies on conceptus regulation of the maternal proinflammatory response to endometrial attachment. Following elongation, pig conceptuses secrete interferon gamma (IFNG) d
Autor:
George C. Gabriel, William A. Devine, Bethany K. Redel, Kristin M. Whitworth, Melissa Samuel, Lee D. Spate, Raissa F. Cecil, Randall S. Prather, Yijen L. Wu, Kevin D. Wells, Cecilia W. Lo
Publikováno v:
Scientific Reports. 12
The pig is an ideal model system for studying human development and disease due to its similarities to human anatomy, physiology, size, and genome. Further, advances in CRISPR gene editing have made genetically engineered pigs viable models for the s
Publikováno v:
Molecular Reproduction and Development
Glutamine supplementation to porcine embryo culture medium improves development, increases leucine consumption, and enhances mitochondrial activity. In cancer cells, glutamine has been implicated in the activation of mechanistic target of rapamycin c
Autor:
Paula R Chen, Randall S. Prather, Joshua A. Benne, Lee D. Spate, Taylor K. Hord, Raissa F. Cecil, Melissa Samuel
Publikováno v:
Molecular Reproduction and Development
To improve efficiency of somatic cell nuclear transfer (SCNT), it is necessary to modify differentiated donor cells to become more amendable for reprogramming by the oocyte cytoplasm. A key feature that distinguishes somatic/differentiated cells from