Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies.

Autor: Lee A Armstrong, Sven M Lange, Virginia Dee Cesare, Stephen P Matthews, Raja Sekhar Nirujogi, Isobel Cole, Anthony Hope, Fraser Cunningham, Rachel Toth, Rukmini Mukherjee, Denisa Bojkova, Franz Gruber, David Gray, Paul G Wyatt, Jindrich Cinatl, Ivan Dikic, Paul Davies, Yogesh Kulathu

Publikováno v: PLoS ONE, Vol 16, Iss 7, p e0253364 (2021)

Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize. Encoded

Externí odkaz: https://doaj.org/article/7e4e63d2ba344215bb01a64466ff2cc4

Comprehensive approach to study branched ubiquitin chains reveals roles for K48-K63 branches in VCP/p97-related processes

Autor: Sven M. Lange, Matthew R. McFarland, Frederic Lamoliatte, Dominika Kwaśna, Linnan Shen, Iona Wallace, Isobel Cole, Lee A. Armstrong, Axel Knebel, Clare Johnson, Virginia De Cesare, Yogesh Kulathu

Branched ubiquitin (Ub) chains make up a significant proportion of Ub polymers in human cells and are formed when two or more sites on a single Ub molecule are modified with Ub creating bifurcated architectures. Despite their abundance, we have a poo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e35913596d7c4ef61fc79481ed7099a7
https://doi.org/10.1101/2023.01.10.523363

Mechanism of activation and regulation of deubiquitinase activity in MINDY1 and MINDY2

Autor: Sven M. Lange, Syed Arif Abdul Rehman, Dmitri I. Svergun, Lee A. Armstrong, Yogesh Kulathu, Yosua Adi Kristariyanto, Axel Knebel, Tobias W. Gräwert

Publikováno v: 'Molecular Cell ', vol: 81, pages: 4176-4190 (2021)
Molecular cell 81, S1097276521006912 (1-15) (2021). doi:10.1016/j.molcel.2021.08.024
Molecular Cell

Summary Of the eight distinct polyubiquitin (polyUb) linkages that can be assembled, the roles of K48-linked polyUb (K48-polyUb) are the most established, with K48-polyUb modified proteins being targeted for degradation. MINDY1 and MINDY2 are members

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b23d77ec8d443d9dee20b6661b5b4428
https://epn-library.esrf.fr/flora/jsp/index_view_direct_anonymous.jsp?record=doc:PUB_ESRF:58802

Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies

Autor: Ivan Dikic, Rachel Toth, Sven M. Lange, Stephen P. Matthews, Isobel Cole, Paul G. Wyatt, Virginia Dee Cesare, Franz S. Gruber, Anthony G. Hope, Fraser Cunningham, Lee A. Armstrong, Denisa Bojkova, Yogesh Kulathu, Rukmini Mukherjee, Raja Sekhar Nirujogi, David W. Gray, Paul Davies, Jindrich Cinatl

Publikováno v: PLoS ONE, Vol 16, Iss 7, p e0253364 (2021)
PLoS ONE

Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is the largest and plays important roles in the viral life cycle. Being a large, multidomain, transmembrane protein, Nsp3 has been the most challenging Nsp to characterize. Encoded

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bd32b1ded91543c9a2151942185c437
https://doaj.org/article/7e4e63d2ba344215bb01a64466ff2cc4

Structural basis for DNA 3'-end processing by human tyrosyl-DNA phosphodiesterase 1

Autor: Fiona J, Flett, Emilija, Ruksenaite, Lee A, Armstrong, Shipra, Bharati, Roberta, Carloni, Elizabeth R, Morris, C Logan, Mackay, Heidrun, Interthal, Julia M, Richardson

Publikováno v: Nature Communications

Tyrosyl-DNA phosphodiesterase (Tdp1) is a DNA 3′-end processing enzyme that repairs topoisomerase 1B-induced DNA damage. We use a new tool combining site-specific DNA–protein cross-linking with mass spectrometry to identify Tdp1 interactions with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::48dec214d00824c13c0ed0e3bc8990f6
https://pubmed.ncbi.nlm.nih.gov/29295983