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High-throughput in vivo mapping of RNA accessible interfaces to identify functional sRNA binding sites.

Autor: Mihailovic MK; McKetta Department of Chemical Engineering, University of Texas at Austin, 200 E. Dean Keeton St., Stop C0400, Austin, TX, 78712, USA., Vazquez-Anderson J; McKetta Department of Chemical Engineering, University of Texas at Austin, 200 E. Dean Keeton St., Stop C0400, Austin, TX, 78712, USA., Li Y; Department of Operations Research and Financial Engineering, Princeton University, Sherrerd Hall, Charlton St., Princeton, NJ, 08544, USA., Fry V; McKetta Department of Chemical Engineering, University of Texas at Austin, 200 E. Dean Keeton St., Stop C0400, Austin, TX, 78712, USA., Vimalathas P; McKetta Department of Chemical Engineering, University of Texas at Austin, 200 E. Dean Keeton St., Stop C0400, Austin, TX, 78712, USA., Herrera D; Department of Computer Science, University of Texas at Austin, 2317 Speedway Stop D9500, Austin, TX, 78712, USA., Lease RA; Department of Chemical and Biomolecular Engineering, The Ohio State University, 151W. Woodruff Ave, Columbus, OH, 43210, USA.; Department of Chemistry and Biochemistry, The Ohio State University, 100W. 18th Ave, Columbus, OH, 43210, USA., Powell WB; Department of Operations Research and Financial Engineering, Princeton University, Sherrerd Hall, Charlton St., Princeton, NJ, 08544, USA., Contreras LM; McKetta Department of Chemical Engineering, University of Texas at Austin, 200 E. Dean Keeton St., Stop C0400, Austin, TX, 78712, USA. lcontrer@che.utexas.edu.

Publikováno v: Nature communications [Nat Commun] 2018 Oct 04; Vol. 9 (1), pp. 4084. Date of Electronic Publication: 2018 Oct 04.

A Modular Genetic System for High-Throughput Profiling and Engineering of Multi-Target Small RNAs.

Autor: Stimple SD; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA., Lahiry A; Department of Microbiology, The Ohio State University, Columbus, OH, USA., Taris JE; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA., Wood DW; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA.; Department of Microbiology, The Ohio State University, Columbus, OH, USA., Lease RA; William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH, USA. lease.22@osu.edu.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, USA. lease.22@osu.edu.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1737, pp. 373-391.