Proteome-scale characterisation of motif-based interactome rewiring by disease mutations

Autor: Johanna Kliche, Leandro Simonetti, Izabella Krystkowiak, Hanna Kuss, Marcel Diallo, Emma Rask, Jakob Nilsson, Norman E Davey, Ylva Ivarsson

Publikováno v: Molecular Systems Biology, Vol 20, Iss 9, Pp 1025-1048 (2024)

Abstract Whole genome and exome sequencing are reporting on hundreds of thousands of missense mutations. Taking a pan-disease approach, we explored how mutations in intrinsically disordered regions (IDRs) break or generate protein interactions mediat

Externí odkaz: https://doaj.org/article/0bbc2585d8e5430193b425c67f6f3b23

Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets

Autor: Filip Mihalič, Caroline Benz, Eszter Kassa, Richard Lindqvist, Leandro Simonetti, Raviteja Inturi, Hanna Aronsson, Eva Andersson, Celestine N. Chi, Norman E. Davey, Anna K. Överby, Per Jemth, Ylva Ivarsson

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-18 (2023)

Abstract The virus life cycle depends on host-virus protein-protein interactions, which often involve a disordered protein region binding to a folded protein domain. Here, we used proteomic peptide phage display (ProP-PD) to identify peptides from th

Externí odkaz: https://doaj.org/article/0dc8318509ba40b4a7a4978a68d342a6

Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs

Autor: Filip Mihalič, Leandro Simonetti, Girolamo Giudice, Marie Rubin Sander, Richard Lindqvist, Marie Berit Akpiroro Peters, Caroline Benz, Eszter Kassa, Dilip Badgujar, Raviteja Inturi, Muhammad Ali, Izabella Krystkowiak, Ahmed Sayadi, Eva Andersson, Hanna Aronsson, Ola Söderberg, Doreen Dobritzsch, Evangelia Petsalaki, Anna K. Överby, Per Jemth, Norman E. Davey, Ylva Ivarsson

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-20 (2023)

Abstract Viruses mimic host short linear motifs (SLiMs) to hijack and deregulate cellular functions. Studies of motif-mediated interactions therefore provide insight into virus-host dependencies, and reveal targets for therapeutic intervention. Here,

Externí odkaz: https://doaj.org/article/e64e95cb96fe4ac196aaf0a9198064f8

Large scale discovery of coronavirus-host factor protein interaction motifs reveals SARS-CoV-2 specific mechanisms and vulnerabilities

Autor: Thomas Kruse, Caroline Benz, Dimitriya H. Garvanska, Richard Lindqvist, Filip Mihalic, Fabian Coscia, Raviteja Inturi, Ahmed Sayadi, Leandro Simonetti, Emma Nilsson, Muhammad Ali, Johanna Kliche, Ainhoa Moliner Morro, Andreas Mund, Eva Andersson, Gerald McInerney, Matthias Mann, Per Jemth, Norman E. Davey, Anna K. Överby, Jakob Nilsson, Ylva Ivarsson

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

Many interactions between viral and host proteins are mediated by short peptide motifs. Here, using a phage-based viral peptide library, the authors identify 269 peptide-based interactions for 18 coronaviruses, including an interaction between SARS-C

Externí odkaz: https://doaj.org/article/1f444706eff944aca145f8ed28412f90

SLiM-binding pockets: an attractive target for broad-spectrum antivirals

Autor: Leandro Simonetti, Jakob Nilsson, Gerald McInerney, Ylva Ivarsson, Norman E. Davey

Publikováno v: Simonetti, L, Nilsson, J, McInerney, G, Ivarsson, Y & Davey, N E 2023, ' SLiM-binding pockets : an attractive target for broad-spectrum antivirals ', Trends in Biochemical Sciences, vol. 48, no. 5, pp. 420-427 . https://doi.org/10.1016/j.tibs.2022.12.004

Short linear motif (SLiM)-mediated interactions offer a unique strategy for viral intervention due to their compact interfaces, ease of convergent evolution, and key functional roles. Consequently, many viruses extensively mimic host SLiMs to hijack

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43ba4534b2175a3c0dcf89dd339f00d7
https://doi.org/10.1016/j.tibs.2022.12.004