Computationally designed mRNA-launched protein nanoparticle vaccines.

Autor: Hendricks GG; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Grigoryan L; Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Navarro MJ; Department of Biochemistry, University of Washington, Seattle, WA, USA., Catanzaro NJ; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Hubbard ML; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Powers JM; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Mattocks M; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Treichel C; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Walls AC; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Howard Hughes Medical Institute, Seattle, WA, USA., Lee J; Department of Biochemistry, University of Washington, Seattle, WA, USA., Ellis D; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Wang JYJ; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Cheng S; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Miranda MC; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Valdez A; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Chao CW; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, WA, USA., Chan S; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Men C; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Johnson MR; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Hui H; Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Wu SY; Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Lujan V; Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Muramatsu H; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Lin PJC; Acuitas Therapeutics, Vancouver, BC, Canada., Sung MMH; Acuitas Therapeutics, Vancouver, BC, Canada., Tam YK; Acuitas Therapeutics, Vancouver, BC, Canada., Leaf EM; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA., Pardi N; Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Pulendran B; Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford University, Stanford, CA, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., King NP; Institute for Protein Design, University of Washington, Seattle, WA, USA.; Department of Biochemistry, University of Washington, Seattle, WA, USA.; Lead contact.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 23. Date of Electronic Publication: 2024 Jul 23.

Protein nanoparticle vaccines induce potent neutralizing antibody responses against MERS-CoV.

Autor: Chao CW; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA., Sprouse KR; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Miranda MC; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Catanzaro NJ; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Hubbard ML; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Addetia A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Stewart C; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Brown JT; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Dosey A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Valdez A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Ravichandran R; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Hendricks GG; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Ahlrichs M; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Dobbins C; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Hand A; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Treichel C; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Willoughby I; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Walls AC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., McGuire AT; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Leaf EM; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Schäfer A; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.; Howard Hughes Medical Institute, Seattle, WA 98195, USA., King NP; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA.; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 14. Date of Electronic Publication: 2024 Mar 14.

Broad receptor tropism and immunogenicity of a clade 3 sarbecovirus.

Autor: Lee J; Department of Biochemistry, University of Washington, Seattle, WA, USA., Zepeda SK; Department of Biochemistry, University of Washington, Seattle, WA, USA., Park YJ; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA., Taylor AL; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Quispe J; Department of Biochemistry, University of Washington, Seattle, WA, USA., Stewart C; Department of Biochemistry, University of Washington, Seattle, WA, USA., Leaf EM; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Treichel C; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Corti D; Humabs Biomed SA, a Subsidiary of Vir. Biotechnology, 6500 Bellinzona, Switzerland., King NP; Department of Biochemistry, University of Washington, Seattle, WA, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Starr TN; Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA., Veesler D; Department of Biochemistry, University of Washington, Seattle, WA, USA; Howard Hughes Medical Institute, Seattle, WA 98195, USA. Electronic address: dveesler@uw.edu.

Publikováno v: Cell host & microbe [Cell Host Microbe] 2023 Dec 13; Vol. 31 (12), pp. 1961-1973.e11. Date of Electronic Publication: 2023 Nov 20.

Vaccination with a structure-based stabilized version of malarial antigen Pfs48/45 elicits ultra-potent transmission-blocking antibody responses.

Autor: McLeod B; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada., Mabrouk MT; Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA., Miura K; Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, USA., Ravichandran R; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Kephart S; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Hailemariam S; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada., Pham TP; Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 12735 Twinbrook Parkway, Rockville, MD 20852, USA., Semesi A; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada., Kucharska I; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada., Kundu P; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada., Huang WC; Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA., Johnson M; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Blackstone A; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Pettie D; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Murphy M; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Kraft JC; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Leaf EM; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Jiao Y; Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA., van de Vegte-Bolmer M; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands., van Gemert GJ; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands., Ramjith J; Radboud Institute for Health Sciences, Department for Health Evidence, Biostatistics Section, Radboud University Medical Center, Nijmegen, the Netherlands., King CR; PATH's Malaria Vaccine Initiative, 455 Massachusetts Avenue NW Suite 1000, Washington, DC 20001, USA., MacGill RS; PATH's Malaria Vaccine Initiative, 455 Massachusetts Avenue NW Suite 1000, Washington, DC 20001, USA., Wu Y; PATH's Malaria Vaccine Initiative, 455 Massachusetts Avenue NW Suite 1000, Washington, DC 20001, USA., Lee KK; Department of Medicinal Chemistry, University of Washington, Seattle, WA 98195, USA., Jore MM; Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, the Netherlands., King NP; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Institute for Protein Design, University of Washington, Seattle, WA 98195, USA., Lovell JF; Department of Biomedical Engineering, University at Buffalo, State University of New York, Buffalo, NY 14260, USA., Julien JP; Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 686 Bay Street, Toronto, ON M5G 0A4, Canada; Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada; Department of Immunology, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada. Electronic address: jean-philippe.julien@sickkids.ca.

Publikováno v: Immunity [Immunity] 2022 Sep 13; Vol. 55 (9), pp. 1680-1692.e8. Date of Electronic Publication: 2022 Aug 16.

Role of Runx2 in Calcific Aortic Valve Disease in Mouse Models.

Autor: Dharmarajan S; Department of Bioengineering, University of Washington, Seattle, WA, United States., Speer MY; Department of Bioengineering, University of Washington, Seattle, WA, United States., Pierce K; Department of Bioengineering, University of Washington, Seattle, WA, United States., Lally J; Department of Bioengineering, University of Washington, Seattle, WA, United States., Leaf EM; Department of Bioengineering, University of Washington, Seattle, WA, United States., Lin ME; Department of Bioengineering, University of Washington, Seattle, WA, United States., Scatena M; Department of Bioengineering, University of Washington, Seattle, WA, United States., Giachelli CM; Department of Bioengineering, University of Washington, Seattle, WA, United States.

Publikováno v: Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2021 Oct 29; Vol. 8, pp. 687210. Date of Electronic Publication: 2021 Oct 29 (Print Publication: 2021).