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Alternative Assay Reagents for UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB Module

Autor: Felix Kaspar, Charity S. G. Ganskow, Lea Eilert, Philipp Klahn, Anett Schallmey

Publikováno v: Analytical Chemistry. 94:8132-8135

This report advises against the use of 5-iodoridine or 5-ethynyluridine as alternative assay reagents in the PUB module, primarily due to their lack of an isosbestic point of phosphorolysis under moderately alkaline conditions.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c410c4b71b1eda10530cc635ee108c1e
https://doi.org/10.1021/acs.analchem.2c01404

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Biased Borate Esterification during Nucleoside Phosphorylase‐Catalyzed Reactions: Apparent Equilibrium Shifts and Kinetic Implications**

Autor: Felix Kaspar, Felix Brandt, Sarah Westarp, Lea Eilert, Sebastian Kemper, Anke Kurreck, Peter Neubauer, Christoph R. Jacob, Anett Schallmey

Publikováno v: Angewandte Chemie International Edition. 62

Biocatalytic nucleoside (trans-)glycosylations catalyzed by nucleoside phosphorylases have graduated to a practical and convenient approach to the preparation of modified nucleosides, which are important pharmaceuticals for the treatment of various c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50212f94cb71fd607f542714463e0354
https://doi.org/10.1002/anie.202218492

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Unusual DNA-binding properties of the Arabidopsis thaliana WRKY50 transcription factor at target gene promoters

Autor: Konstantin Kanofsky, Fabian Machens, Reinhard Hehl, Jendrik Rusche, Lea Eilert

Publikováno v: Plant Cell Reports

Key message WRKY50 from A. thaliana requires WT-boxes at target gene promoters for activation and binding. Abstract Based on the genome-wide prediction of WRKY50 target genes and the similarity of a WRKY50 binding site to WT-boxes in microbe-associat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::37467b7a41704b5070810c1a06aa4327
http://europepmc.org/articles/PMC7811519

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UV-Spectroscopic Detection of (Pyro-)Phosphate with the PUB module

Autor: Lea Eilert, Anett Schallmey, Felix Kaspar

Despite the prevalence of ortho- and pyrophosphate in biochemistry, operationally simple and versatile high-throughput methodologies for their quantification are lacking. We herein introduce PUB, a module for phosphate detection by continuous UV-spec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28db3fcceaa7ddf6dfc2856d61980782
https://doi.org/10.26434/chemrxiv-2021-846w3

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