Zobrazeno 1 - 10
of 10
pro vyhledávání: '"León F. Garcı́a-Martı́nez"'
Cyclin T1 domains involved in complex formation with tat and TAR RNA are critical for tat-activation
Autor:
Jun Guo, Youn Tae Kwak, Richard B. Gaynor, Dmitri Ivanov, Eugene Nee, León F. Garcı́a-Martı́nez
Publikováno v:
Journal of Molecular Biology. 288:41-56
Tat activates transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) by increasing the processivity of RNA polymerase II. Recently, it has been demonstrated that the cellular kinase CDK9 and its binding partner
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bde8d6fa4fbec86c988caee8e1e63e8
https://doi.org/10.1006/jmbi.1999.2663
https://doi.org/10.1006/jmbi.1999.2663
DNA-Dependent Protein Kinase Phosphorylation of IκBα and IκBβ Regulates NF-κB DNA Binding Properties
Autor:
Li Liu, Xiao Hua Li, León F. Garcı́a-Martı́nez, William S. Lane, Katheryn Meek, Youn Tae Kwak, Richard B. Gaynor, F Bex
Publikováno v:
Molecular and Cellular Biology. 18:4221-4234
Regulation of the IκBα and IκBβ proteins is critical for modulating NF-κB-directed gene expression. Both IκBα and IκBβ are substrates for cellular kinases that phosphorylate the amino and carboxy termini of these proteins and regulate their
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fb9774ebee7d5fe472df0df0faabd7cd
https://doi.org/10.1128/mcb.18.7.4221
https://doi.org/10.1128/mcb.18.7.4221
Publikováno v:
The EMBO Journal. 16:1224-1235
The ability of human immunodeficiency virus-1 (HIV-1) to undergo efficient reverse transcription is dependent on a number of parameters. These include the binding of the tRNA(3)(Lys) to the HIV-1 primer binding site and the subsequent interaction wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::022ccfcdabfd54c0002f4385133cd91d
https://doi.org/10.1093/emboj/16.6.1224
https://doi.org/10.1093/emboj/16.6.1224
Publikováno v:
Journal of Biological Chemistry. 272:6951-6958
The HIV-1 (human immunodeficiency virus type 1) and HIV-2 Tat proteins increase the level of transcription from their corresponding long terminal repeats. Tat activates transcription likely by interaction with components of the transcriptional initia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a91c99b1ec9d7118ac5b57c283db4a8b
https://doi.org/10.1074/jbc.272.11.6951
https://doi.org/10.1074/jbc.272.11.6951
Autor:
Gopinath Mavankal, Richard B. Gaynor, Michael J. Orsini, Christine Debouck, León F. Garcı́a-Martı́nez
Publikováno v:
Protein Expression and Purification. 8:238-246
We describe a single method to purify milligram amounts of authentic wild-type and mutant HIV-1 and HIV-2 Tat proteins overexpressed in Escherichia coli. This method takes advantage of the highly basic, positively charged RNA binding domain present i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b21b0bc2d5b181d3d94178680dda8c44
https://doi.org/10.1006/prep.1996.0096
https://doi.org/10.1006/prep.1996.0096
Publikováno v:
Scopus-Elsevier
Human immunodeficiency virus type 1 (HIV-1) gene expression is modulated by both viral and cellular factors. A regulatory element in the HIV-1 long terminal repeat known as TAR, which extends from nucleotides -18 to +80, is critical for the activatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b2ab1d465c75feb5a8fc08bda082cb6
https://doi.org/10.1128/jvi.69.6.3584-3596.1995
https://doi.org/10.1128/jvi.69.6.3584-3596.1995
Publikováno v:
Journal of Virology. 68:7188-7199
Human immunodeficiency virus type 1 (HIV-1) gene expression is dependent on a number of cis-acting DNA elements present in the HIV-1 long terminal repeat. Previous studies have demonstrated that the TATA element is critical for basal and Tat-induced
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90a763abbde34769fa7fee64801954dc
https://doi.org/10.1128/jvi.68.11.7188-7199.1994
https://doi.org/10.1128/jvi.68.11.7188-7199.1994
Autor:
León F. Garcı́a-Martı́nez, Graham K. Bilter, Robert Kovelman, Miguel S. Barbosa, Jun Wu, Joe O'Neill
Publikováno v:
Analytical biochemistry. 301(1)
We developed a 96-well microtiter-plate high-throughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6b08c03de6b173b113da62a5ccf26b3
https://pubmed.ncbi.nlm.nih.gov/11811973
https://pubmed.ncbi.nlm.nih.gov/11811973
Autor:
Dmitri Ivanov, Gopinath Mavankal, Richard B. Gaynor, John M. Neveu, León F. Garcı́a-Martı́nez, William S. Lane
The Tat protein is a transcriptional activator which is required for efficient human immunodeficiency virus 1 (HIV-1) gene expression Tat stimulates HIV-1 transcriptional elongation by increasing the processivity of RNA polymerase II. To address whet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a493c6d2312e11893aef133666684ccd
https://europepmc.org/articles/PMC1169892/
https://europepmc.org/articles/PMC1169892/
Autor:
Richard B. Gaynor, León F. Garcı́a-Martı́nez, Foon Wu-Baer, Pierette Peters, Gopinath Mavankal
Publikováno v:
Journal of molecular biology. 254(3)
In this study we have defined the in vitro requirements for transcriptional regulation of the HIV-2 LTR in response to the HIV-1 and HIV-2 Tat proteins and addressed potential mechanisms of Tat function. HIV-2 contains a duplicated TAR RNA stem-loop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3b5564a2699d1db0f7a4639b2d84981
https://pubmed.ncbi.nlm.nih.gov/7490754
https://pubmed.ncbi.nlm.nih.gov/7490754