Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Layla Fernández"'
Autor:
Manfred T. Reetz, Luciana G. de Oliveira, Layla Fernández, Pankaj Soni, Ning Jiao, Yosephine Gumulya
Publikováno v:
Biocatalysis and Biotransformation. 28:122-129
The yeast Pichia pastoris is being increasingly used as a host for expressing enzymes on a large scale, but application in directed evolution requiring efficient expression of libraries of mutants is hampered due to the time-consuming multistep proce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbc9f74be30dfdd2c012776b9169f3a6
https://doi.org/10.3109/10242420903505834
https://doi.org/10.3109/10242420903505834
Autor:
Horst Höbenreich, Sheng Wu, Sabrina Kille, Renate Lohmer, Felipe E. Zilly, Shreenath Prasad, Jérôme J.-P. Peyralans, Andreas Taglieber, Yosephine Gumulya, Daniel Kahakeaw, Jullien Drone, John Podtetenieff, J. Daniel Carballeira, Joaquin Sanchis, Manfred T. Reetz, Layla Fernández, Pankaj Soni
Publikováno v:
Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, Springer Verlag, 2008, 81, pp.387-397. ⟨10.1007/s00253-008-1678-9⟩
Applied Microbiology and Biotechnology, Springer Verlag, 2008, 81, pp.387-397. ⟨10.1007/s00253-008-1678-9⟩
International audience; Saturation mutagenesis constitutes a powerful method in the directed evolution of enzymes. Traditional protocols of whole plasmid amplification such as Stratagene's QuikChange sometimes fail when the templates are difficult to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3eb1acbbd27e618aa874f76a63f5fc86
https://doi.org/10.1007/s00253-008-1678-9
https://doi.org/10.1007/s00253-008-1678-9
Autor:
Oscar Bañuelos, Javier Velasco, José M. Corral, Layla Fernández, Magdalena Valdivieso-Ugarte, José L. Adrio
Publikováno v:
Anaerobe. 14:184-189
The capacity of two probiotic strains, isolated from human breast milk, to use several beta(2-1) fructan mixtures as carbon and energy source in in vitro cultures has been tested. Results showed that both strains, Lactobacillus gasseri CECT5714 and L
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc4f67fba7bc26da2e4dc767c069c629
https://doi.org/10.1016/j.anaerobe.2008.02.002
https://doi.org/10.1016/j.anaerobe.2008.02.002
Autor:
Shyrley Paola Amarilla, Belén Huerta, Layla Fernández, Jaime Gómez-Laguna, Lidia Gómez-Gascón, Fernando Cardoso-Toset, Pilar Ruiz, Inmaculada Luque, Librado Carrasco
Publikováno v:
The Veterinary Journal 204, 232-234 (2015)
Helvia. Repositorio Institucional de la Universidad de Córdoba
instname
Helvia. Repositorio Institucional de la Universidad de Córdoba
instname
Free-range pigs can be infected by Mycobacterium tuberculosis complex (MTC) and may contribute to the spread of bovine tuberculosis (bTB). In the present study, the diagnostic values of bacteriological culture, a duplex real-time quantitative PCR and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01fea272739248e8e2402d5b5b70c6c6
https://hdl.handle.net/10396/15786
https://hdl.handle.net/10396/15786
Autor:
Alberto Zafra, Javier Velasco, Pedro L. Benítez, Layla Fernández, José L. Adrio, Juan Carlos Morales, Ignacio Pérez-Victoria
Publikováno v:
Protein expression and purification. 49(2)
The mature lipI gene, encoding the lipase I from Galactomyces geotrichum BT107, was obtained by PCR from genomic DNA, sequenced and cloned into a Pichia pastoris expression vector. Clones containing multiple copies of lipI integrated in their genome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da5423d6abc2ef22a70727567facf218
https://pubmed.ncbi.nlm.nih.gov/16884921
https://pubmed.ncbi.nlm.nih.gov/16884921
Publikováno v:
Chemical Communications. 46:8657
Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc09bf3b50b32886e35ad276424164ac
https://doi.org/10.1039/c0cc02657c
https://doi.org/10.1039/c0cc02657c
Publikováno v:
Chemical Communications. :5502
As an alternative to screening in the directed evolution of enantioselective enzymes, a selection system has been implemented for a lipase-catalyzed hydrolytic kinetic resolution of a chiral ester.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81d6c3408c019f55b3adbaefa8a45ce7
https://doi.org/10.1039/b814538e
https://doi.org/10.1039/b814538e