Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Lawrence R. Gurley"'
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 712:211-224
The protein kinase inhibitor staurosporine (Stsp) has been used extensively to study physiological functions, biochemical mechanisms, and cancer therapy. Using an HPLC assay for Stsp developed in our laboratory, we find that only 0.7% of Stsp remains
Publikováno v:
Biochemistry. 36:13761-13768
In mammalian cells, up to six serines and threonines in histone H1 are phosphorylated in vivo in a cell cycle dependent manner that has long been linked with chromatin condensation. Growth-associated H1 kinases, now known as cyclin-dependent kinases
Publikováno v:
Methods in Cell Science. 18:115-125
Procedures are described for synchronizing (1) rodent cells in G1 using the isoleucine-deprivation method, (2) normal diploid human and other kinds of fibroblasts in G0 using serum withdrawal, and (3) rodent and human cells in S phase using synchroni
Publikováno v:
Journal of Biological Chemistry. 270:27653-27660
32P-Labeled histone H1 was isolated from synchronized Chinese hamster (line CHO) cells, subjected to tryptic digestion, and fractionated into 15 phosphopeptides by high performance liquid chromatography. These phosphopeptides were grouped into five c
Autor:
T Ellis, W S Session, D. M. Stavert, N. M. Lehnert, Bruce E. Lehnert, Lawrence R. Gurley, D. C. Archuleta
Publikováno v:
Toxicology. 89:239-277
Human inhalation exposures to relatively high mass concentrations of the oxidant gas nitrogen dioxide (NO2) can result in a variety of pulmonary disorders, including life-threatening pulmonary edema, pneumonia, and bronchiolitis obliterans. Inasmuch
Autor:
Lawrence R. Gurley, Lawrence O. Ticknor, M.E. Thompson, Cheryl R. Kuske, Joseph G. Valdez, Paul J. Jackson, E. Guzman
Publikováno v:
Journal of Biological Chemistry. 269:6223-6232
Three isoenzyme forms (designated A, B, and C) of O-acetylserine sulfhydrylase were purified from Datura innoxia suspension cultures. Isoenzyme A is the most abundant form, comprising 45-60% of the total activity. Isoenzymes C and B comprise 35-40% a
Autor:
Joseph G. Valdez, J.A. D'Anna, Theodore T. Puck, Lawrence R. Gurley, R.J. Sebring, A.L. Jandacek
Publikováno v:
Somatic cell and molecular genetics. 24(3)
The proliferation of suspension cultures of malignant CHO cells was inhibited by 0.5 mM Br-cAMP treatment and restored by its removal. This treatment also inhibited histone H1 phosphorylation completely, reduced histones H2A and H4 phosphorylations,
Publikováno v:
The Journal of biological chemistry. 272(44)
Complementation group G of xeroderma pigmentosum (XPG) is one of the most rare and pathophysiologically heterogeneous forms of this inherited disease. XPG patients exhibit varying phenotypes, from having a very mild defect in DNA repair to being seve
Autor:
Bruce E. Lehnert, Lawrence R. Gurley, M. J. Behr, N. M. Lehnert, D. M. Stavert, W S Session, D. C. Archuleta
Publikováno v:
Experimental lung research. 21(2)
Exercise performed after exposure to various pneumoedematogenic gases can increase the severity of pulmonary edema beyond that which occurs when exposure is followed by rest. The present study was performed to investigate the potential relationship b
Publikováno v:
Journal of chromatography. 559(1-2)
Measurements of the biochemical constituents in the fluid lining of the lung can be used for diagnosing and assessing lung disorders. To facilitate such measurements, a high-performance capillary electrophoresis (HPCE) method has been developed by wh