Zobrazeno 1 - 10
of 80
pro vyhledávání: '"Lawrence J. Parkhurst"'
Autor:
Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera, Lawrence J. Parkhurst
Publikováno v:
Biosensors, Vol 10, Iss 11, p 180 (2020)
The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), po
Externí odkaz:
https://doaj.org/article/7dfaeab36ec547de87ec5e260492acf3
Autor:
Roberto F Delgadillo, Timothy C Mueser, Kathia Zaleta-Rivera, Katie A Carnes, José González-Valdez, Lawrence J Parkhurst
Publikováno v:
PLoS ONE, Vol 14, Iss 2, p e0204194 (2019)
The high affinity (KD ~ 10-15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume t
Externí odkaz:
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
Autor:
Katie A. Carnes, Lawrence J. Parkhurst, Kathia Zaleta-Rivera, Omar Olmos, Roberto F. Delgadillo
Publikováno v:
Analytical Chemistry
Time-resolved donor-detected Forster resonance energy transfer (trDDFRET) allows the observation of molecular interactions of dye-labeled biomolecules in the ∼10-100 A region. However, we can observe longer-range interactions when using time-resolv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb2efca23cbd03d418a3d1763c99a910
http://europepmc.org/articles/PMC7992049
http://europepmc.org/articles/PMC7992049
Autor:
Lawrence J. Parkhurst
Publikováno v:
Near-Infrared Applications in Biotechnology
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f4e764d693875d9088d859aaac416728
https://doi.org/10.1201/9780429132650-2
https://doi.org/10.1201/9780429132650-2
Autor:
Timothy C. Mueser, Katie A. Carnes, Lawrence J. Parkhurst, Kathia Zaleta-Rivera, José González-Valdez, Roberto F. Delgadillo
Publikováno v:
PLoS ONE, Vol 14, Iss 2, p e0204194 (2019)
PLoS ONE
PLoS ONE
The high affinity (KD ~ 10−15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73039636a968c86dff7b47ff33d34af0
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
https://doaj.org/article/fb077640e6a6488c9760c8c1768cf7ce
Autor:
Katie A. Carnes, Roberto F. Delgadillo, Kathia Zaleta-Rivera, José González-Valdez, Lawrence J. Parkhurst, Timothy C. Mueser
The high affinity (KD∼ 10−15M) of biotin to avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling. Equilibration times suggested for these assays assume
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85d2c53cabe9fa1a3e9127d0570f7de6
https://doi.org/10.1101/410548
https://doi.org/10.1101/410548
Autor:
Jason G. Glanzer, Shengqin Liu, Lawrence J. Parkhurst, Gregory G. Oakley, Patricia Soto, Katie A. Carnes
Publikováno v:
Nucleic Acids Research
Replication protein A (RPA), essential for DNA replication, repair and DNA damage signalling, possesses six ssDNA-binding domains (DBDs), including DBD-F on the N-terminus of the largest subunit, RPA70. This domain functions as a binding site for p53
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2759bd3a67bcd0426ae19401be622eb6
http://europepmc.org/articles/PMC3561959
http://europepmc.org/articles/PMC3561959
Publikováno v:
Photochemistry and Photobiology. 86:261-272
We report the spectroscopic properties of fluorescein, x-rhodamine, tetramethyl-rhodamine, attached to single strand, duplex DNA, and to the digestion products by DNAse I. The properties reported include: molar absorptivity, quantum yield, absorbance
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::459c82ab27c84ae9c17cffb9c6f36f6e
https://doi.org/10.1111/j.1751-1097.2009.00663.x
https://doi.org/10.1111/j.1751-1097.2009.00663.x
Publikováno v:
Nucleic Acids Research
Local DNA deformation arises from an interplay among sequence-related base stacking, intrastrand phosphate repulsion, and counterion and water distribution, which is further complicated by the approach and binding of a protein. The role of electrosta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60903f2af3d432f2b46bf332aec913a1
http://europepmc.org/articles/PMC1369283
http://europepmc.org/articles/PMC1369283
Publikováno v:
Journal of Biological Chemistry. 276:29782-29791
Binding of the TATA-binding protein (TBP) to promoter DNA bearing the TATA sequence is an obligatory initial step in RNA polymerase II transcription initiation. The interactions of Saccharomyces cerevisiae TBP with the E4 (TATATATA) and adenovirus ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21861e353598f3f472a827abecb97d4b
https://doi.org/10.1074/jbc.m104099200
https://doi.org/10.1074/jbc.m104099200