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Autor:
Wen-Chuan Lee, Lawrence Fu-nien Chu, Teh-yang Huang, Simon J.T. Mao, Redman Chu, Ping-Cheng Yang
Publikováno v:
Protein expression and purification. 10(2)
The 90-kDa heat shock protein (HSP90) was purified from porcine brain by a novel single-step purification procedure using diethylaminoethyl high-performance liquid chromatography (HPLC). About 4.8 mg of HSP90 was isolated from 25 g wet wt porcine bra