Sox2 modulation increases naïve pluripotency plasticity

Autor: Kathryn C. Tremble, Giuliano G. Stirparo, Lawrence E. Bates, Katsiaryna Maskalenka, Hannah T. Stuart, Kenneth Jones, Amanda Andersson-Rolf, Aliaksandra Radzisheuskaya, Bon-Kyoung Koo, Paul Bertone, José C.R. Silva

Publikováno v: iScience, Vol 24, Iss 3, Pp 102153- (2021)

Summary: Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance, and differentiation of naive pluripotent stem cells (nPSCs). Here, we report that self-renewal of nPSCs requires minimal Sox2 expression (Sox2-

Externí odkaz: https://doaj.org/article/0ba31fc74d5742b49d4349e5fd86f383

Tumor suppressors inhibit reprogramming of African spiny mouse ( Acomys) fibroblasts to induced pluripotent stem cells

Autor: Aaron Gabriel W. Sandoval, Malcolm Maden, Lawrence E. Bates, Jose C.R. Silva

Background: The African spiny mouse (Acomys) is an emerging mammalian model for scar-free regeneration, and further study of Acomys could advance the field of regenerative medicine. Isolation of pluripotent stem cells from Acomys would allow for deve

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7955b21d1a2a628f5fc568fbd8211901

OCT4 activates a Suv39h1-repressive antisense lncRNA to couple histone H3 Lysine 9 methylation to pluripotency

Autor: Laure D Bernard, Agnès Dubois, Victor Heurtier, Véronique Fischer, Inma Gonzalez, Almira Chervova, Alexandra Tachtsidi, Noa Gil, Nick Owens, Lawrence E Bates, Sandrine Vandormael-Pournin, José C R Silva, Igor Ulitsky, Michel Cohen-Tannoudji, Pablo Navarro

Publikováno v: Nucleic Acids Research
Nucleic Acids Research, 2022, ⟨10.1093/nar/gkac550⟩
Bernard, L D, Dubois, A, Heurtier, V, Fischer, V, Gonzalez, I, Chervova, A, Tachtsidi, A, Gil, N, Owens, N, Bates, L E, Vandormael-Pournin, S, Silva, J C R, Ulitsky, I, Cohen-Tannoudji, M & Navarro, P 2022, ' OCT4 activates a Suv39h1-repressive antisense lncRNA to couple histone H3 Lysine 9 methylation to pluripotency ', Nucleic Acids Research, vol. 50, no. 13, pp. 7367-7379 . https://doi.org/10.1093/nar/gkac550

Histone H3 Lysine 9 (H3K9) methylation, a characteristic mark of heterochromatin, is progressively implemented during development to contribute to cell fate restriction as differentiation proceeds. Accordingly, in undifferentiated and pluripotent mou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb549b92a0f4c49361d981c802c8fbdd
https://hal-pasteur.archives-ouvertes.fr/pasteur-03719071/file/Bernard_etal_nar_revised_final.pdf

OCT4 induces embryonic pluripotency via STAT3 signaling and metabolic mechanisms

Autor: Lawrence E. Bates, Jennifer Nichols, José C. R. Silva, Thorsten Boroviak, Giuliano Giuseppe Stirparo, Siarhei Hladkou, Agata Kurowski, Hannah T. Stuart, Stanley E. Strawbridge, Ayaka Yanagida

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America

Significance We used single-cell whole-genome transcriptional profiling and protein quantification to investigate the role of OCT4 in establishing pluripotency in the murine embryo. Surprisingly, most pluripotency-associated factors are induced norma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b43e422ba3658d70d42d43bde35188c7
https://doi.org/10.1073/pnas.2008890118

Exit from Naive Pluripotency Induces a Transient X Chromosome Inactivation-like State in Males

Autor: Elsa J, Sousa, Hannah T, Stuart, Lawrence E, Bates, Mohammadmersad, Ghorbani, Jennifer, Nichols, Sabine, Dietmann, José C R, Silva

Publikováno v: Cell Stem Cell

Summary A hallmark of naive pluripotency is the presence of two active X chromosomes in females. It is not clear whether prevention of X chromosome inactivation (XCI) is mediated by gene networks that preserve the naive state. Here, we show that robu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::aa73a619d1cfa074c53af712b0d560a7
https://pubmed.ncbi.nlm.nih.gov/29804891