Zobrazeno 1 - 10
of 181
pro vyhledávání: '"Lawrence M. Schopfer"'
Publikováno v:
PLoS ONE, Vol 19, Iss 4, p e0299701 (2024)
Recombinant Francisella tularensis universal stress protein with a C-terminal histidine-tag (rUsp/His6) was expressed in Escherichia coli. Endogenous F. tularensis Usp has a predicted molecular mass of 30 kDa, but rUsp/His6 had an apparent molecular
Externí odkaz:
https://doaj.org/article/e73ca7c3caa645518287e7eb4d9fe9bb
Publikováno v:
Biochemistry Research International, Vol 2024 (2024)
Bacterial and mammalian cells are rich in putrescine, spermidine, and spermine. Polyamines are required for optimum fitness, but the biological function of these small aliphatic compounds has only been partially revealed. Known functions of polyamine
Externí odkaz:
https://doaj.org/article/b6fb23a9b2624e3bbf65f7519cd577b0
Publikováno v:
PLoS ONE, Vol 18, Iss 1, p e0280380 (2023)
Protection from the toxicity of nerve agents is achieved by pretreatment with human butyrylcholinesterase (BChE). Current methods for purifying large quantities of BChE from frozen Cohn fraction IV-4 produce 99% pure enzyme, but the yield is low (21%
Externí odkaz:
https://doaj.org/article/276bc035f5dc4e2db13f98512aa3fbd3
Publikováno v:
PLoS ONE, Vol 16, Iss 7, p e0254450 (2021)
Isopeptide crosslinked proteins can be the product of transglutaminase or of exposure to organophosphorus toxicants (OP). Transglutaminase links glutamine to lysine with loss of ammonia. OP toxicants induce a link between glutamic acid and lysine wit
Externí odkaz:
https://doaj.org/article/f71f9d78cba4493881187d548b22dde3
Publikováno v:
Data in Brief, Vol 20, Iss , Pp 1607-1619 (2018)
Milk of the domestic pig has 10 times more butyrylcholinesterase (BChE) per mL than porcine serum. We purified BChE from porcine milk by affinity chromatography on Hupresin-Sepharose. The pure porcine BChE (PoBChE) was a tetramer with a molecular wei
Externí odkaz:
https://doaj.org/article/ce29c7e595bb49648548939becf98af0
Publikováno v:
Chemical Research in Toxicology. 35:1570-1578
Exposure to organophosphorus pesticides (OP) can have chronic adverse effects that are independent of inhibition of acetylcholinesterase, the classic target for acute OP toxicity. In pure proteins, the organophosphorus pesticide chlorpyrifos oxon ind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6263da828f1c58ea1652c60c060778ae
https://doi.org/10.1021/acs.chemrestox.2c00194
https://doi.org/10.1021/acs.chemrestox.2c00194
Publikováno v:
Neurotoxicology
Cultured SH-SY5Y human neuroblastoma cells are used in neurotoxicity assays. These cells express markers of the cholinergic and dopaminergic systems. Acetylcholinesterase (AChE) activity has been reported in these cells. Neurotoxic organophosphate co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1c6914486963965bc4cf4fac2371c2d
https://doi.org/10.1016/j.neuro.2022.02.006
https://doi.org/10.1016/j.neuro.2022.02.006
Autor:
Oksana Lockridge, Lawrence M. Schopfer
Publikováno v:
Proceedings, Vol 62, Iss 1, p 5 (2020)
The genes for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) encode the proteins responsible for enzyme activity. Additional gene products, PRiMA and ColQ, anchor AChE and BChE proteins into membranes. Soluble AChE and BChE tetramers ar
Externí odkaz:
https://doaj.org/article/7c7dddfdbd27443fbcb36dbe46ded645
Autor:
Lawrence M. Schopfer, Oksana Lockridge
Publikováno v:
Molecules, Vol 25, Iss 11, p 2659 (2020)
Bacterial transglutaminase was used to label human plasma proteins with fluorescent tags. Protein lysines were modified with dansyl-epsilon-aminohexyl-Gln-Gln-Ile-Val-OH (dansylQQIV), while protein glutamines were modified with dansyl cadaverine. Lab
Externí odkaz:
https://doaj.org/article/6290ada5a55842ac893d4bbd2b28b64a
Publikováno v:
PLoS ONE, Vol 14, Iss 1, p e0209795 (2019)
Human butyrylcholinesterase (HuBChE) is being developed as a therapeutic for protection from the toxicity of nerve agents. An enriched source of HuBChE is Cohn fraction IV-4 from pooled human plasma. For the past 40 years, purification of HuBChE has
Externí odkaz:
https://doaj.org/article/affc078c1bbb4040bab862cd9ed54a21