The alpha/B.1.1.7 SARS-CoV-2 variant exhibits significantly higher affinity for ACE-2 and requires lower inoculation doses to cause disease in K18-hACE2 mice

Autor: Rafael Bayarri-Olmos, Laust Bruun Johnsen, Manja Idorn, Line S Reinert, Anne Rosbjerg, Søren Vang, Cecilie Bo Hansen, Charlotte Helgstrand, Jais Rose Bjelke, Theresa Bak-Thomsen, Søren R Paludan, Peter Garred, Mikkel-Ole Skjoedt

Publikováno v: eLife, Vol 10 (2021)

The alpha/B.1.1.7 SARS-CoV-2 lineage emerged in autumn 2020 in the United Kingdom and transmitted rapidly until winter 2021 when it was responsible for most new COVID-19 cases in many European countries. The incidence domination was likely due to a f

Externí odkaz: https://doaj.org/article/0dacfd22c5c844a6adf835fea181b896

SARS-CoV-2 Neutralizing Antibody Responses towards Full-Length Spike Protein and the Receptor-Binding Domain

Autor: Franziska Zosel, Laust Bruun Johnsen, Jais Rose Bjelke, Charlotte Helgstrand, Joseph G. Jardine, Søren R. Paludan, Manja Idorn, Fredrik Kryh Öberg, Peter Garred, Laura Pérez-Alós, Theresa Bak-Thomsen, Cecilie Bo Hansen, Mikkel-Ole Skjoedt, Max Søgaard, Rafael Bayarri-Olmos, Anne Rosbjerg

Publikováno v: Bayarri-Olmos, R, Idorn, M, Rosbjerg, A, Pérez-Alós, L, Hansen, C B, Johnsen, L B, Helgstrand, C, Zosel, F, Bjelke, J R, Öberg, F K, Søgaard, M, Paludan, S R, Bak-Thomsen, T, Jardine, J G, Skjoedt, M O & Garred, P 2021, ' SARS-CoV-2 neutralizing antibody responses towards full-length spike protein and the receptor-binding domain ', Journal of Immunology, vol. 207, no. 3, pp. 878-887 . https://doi.org/10.4049/jimmunol.2100272

Tools to monitor SARS-CoV-2 transmission and immune responses are needed. We present a neutralization ELISA to determine the levels of Ab-mediated virus neutralization and a preclinical model of focused immunization strategy. The ELISA is strongly co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1e57001fe7043b8ae4c5af67c3a6cd3
https://doi.org/10.4049/jimmunol.2100272

The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization

Autor: Rafael Bayarri-Olmos, Mikkel-Ole Skjoedt, Peter Garred, Anne Rosbjerg, Laust Bruun Johnsen, Theresa Bak-Thomsen, Charlotte Helgstrand

SARS-CoV-2 transmission from humans to animals has been reported for many domesticated species, including cats, dogs and minks. Identification of novel spike gene mutations appearing in minks has raised major concerns about potential immune evasion a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b3c8a12acc9780b8e588f1877def93cc
https://doi.org/10.1101/2021.01.29.428834

Reply to Lassaunière:On the functional characterization of the Y453F RBD variant found in cluster 5 SARS-CoV-2

Autor: Laust Bruun Johnsen, Rafael Bayarri-Olmos, Anne Rosbjerg, Charlotte Helgstrand, Peter Garred, Mikkel-Ole Skjoedt, Theresa Bak-Thomsen

Publikováno v: Bayarri-Olmos, R, Johnsen, L B, Rosbjerg, A, Helgstrand, C, Bak-Thomsen, T, Garred, P & Skjoedt, M O 2021, ' Reply to Lassaunière : On the functional characterization of the Y453F RBD variant found in cluster 5 SARS-CoV-2 ', Journal of Biological Chemistry, vol. 297, no. 5, 101241 . https://doi.org/10.1016/j.jbc.2021.101241

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae6e0810909e0a3084ecfcbc28c84118
https://curis.ku.dk/portal/da/publications/reply-to-lassauniere(d5aedd8f-fb2b-4416-b644-de6f550f5b18).html

Optimisation of the Factor VIII yield in mammalian cell cultures by reducing the membrane bound fraction

Autor: Mille Kolind, Martin W. Berchtold, Peder Lisby Nørby, Laust Bruun Johnsen

Publikováno v: Journal of Biotechnology. 151:357-362

In vivo, clotting Factor VIII (FVIII) circulates in plasma bound to von Willebrand factor (vWF), and the vWF:FVIII complex prevents binding of FVIII to phosphatidylserine (PS). Activation of FVIII by thrombin releases FVIII from vWF, and subsequently

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::457a61a7d855cbd443a61ac5ea9d25d1
https://doi.org/10.1016/j.jbiotec.2010.12.019