Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Laurie Lamoreaux"'
Autor:
Richard A Koup, Mario Roederer, Laurie Lamoreaux, Jennifer Fischer, Laura Novik, Martha C Nason, Brenda D Larkin, Mary E Enama, Julie E Ledgerwood, Robert T Bailer, John R Mascola, Gary J Nabel, Barney S Graham, VRC 009 Study Team, VRC 010 Study Team
Publikováno v:
PLoS ONE, Vol 5, Iss 2, p e9015 (2010)
Induction of HIV-1-specific T-cell responses relevant to diverse subtypes is a major goal of HIV vaccine development. Prime-boost regimens using heterologous gene-based vaccine vectors have induced potent, polyfunctional T cell responses in preclinic
Externí odkaz:
https://doaj.org/article/d38547ce8a244bc9a6f7395ca975d051
Autor:
Nancy J Sullivan, Thomas W Geisbert, Joan B Geisbert, Devon J Shedlock, Ling Xu, Laurie Lamoreaux, Jerome H H V Custers, Paul M Popernack, Zhi-Yong Yang, Maria G Pau, Mario Roederer, Richard A Koup, Jaap Goudsmit, Peter B Jahrling, Gary J Nabel
Publikováno v:
PLoS Medicine, Vol 3, Iss 6, p e177 (2006)
Ebola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for Ebola virus infection, the development of a vaccine becomes an important strategy to contain outbreaks. Immuni
Externí odkaz:
https://doaj.org/article/58829cdaa1924306b3ac4ae365094197
Autor:
Pratip K. Chattopadhyay, Andrew McDavid, Laurie Lamoreaux, Richard A. Koup, Maria Dominguez, Raphael Gottardo, Greg Finak, Mario Roederer, Steven Ma
Publikováno v:
Journal of Immunological Methods. 391:133-145
Highly multiplexed, single-cell technologies reveal important heterogeneity within cell populations. Recently, technologies to simultaneously measure expression of 96 (or more) genes from a single cell have been developed for immunologic monitoring.
Autor:
Michael R. Betts, Julia A. Metcalf, Laurie Lamoreaux, Elizabeth Urban, Christian Yoder, Julie Gumkowski, Marybeth Daucher, Daniel C. Douek, Jason M. Brenchley, Catherine Rehm, Elizabeth Nies-Kraske, Mark Dybul, David Price, Diane Rock
Publikováno v:
Journal of Virology. 82:4102-4114
A clear understanding of the antiviral effects of CD8+T cells in the context of chronic human immunodeficiency virus (HIV) infection is critical for the development of prophylactic vaccines and therapeutics designed to support T-cell-mediated immunit
Publikováno v:
Nature Protocols. 1:1507-1516
We describe here a method for optimizing the use of polychromatic flow cytometry (with up to 17 fluorochromes simultaneously) in surface and intracellular staining of human T lymphocytes. We will highlight and discuss how to procedurally optimize key
Autor:
David R. Ambrozak, Mario Roederer, Pratip K. Chattopadhyay, Richard A. Koup, Richard Nguyen, Laurie Lamoreaux, Stephen P. Perfetto
Publikováno v:
Journal of Immunological Methods. 313:199-208
Membrane-damaged cells caused by either mechanical trauma or through normal biological processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead cells can nonspecifically bind monoclonal antibody conjugates, potentially le
Autor:
James J. Brendle, Laurie Lamoreaux, Steve Perfetto, Courtney G Havens, Karl A. Werbovetz, Ludmila V. Asher, Nelva Bryant
Publikováno v:
Molecular and Biochemical Parasitology. 110:223-236
To aid our investigation of tubulin as an antileishmanial drug target, the effects of the mammalian antimicrotubule agents ansamitocin P3, taxol, and hemiasterlin on Leishmania donovani promastigotes were described. These drugs affected the assembly
Autor:
Pratip K. Chattopadyay, Laurie Lamoreaux, Steven S. Ma, Maria Dominguez, Greg Finak, Mario Roederer, Raphael Gottardo, Andrew McDavid
Publikováno v:
Bioinformatics
Cell populations are never truly homogeneous; individual cells exist in biochemical states that define functional differences between them. New technology based on microfluidic arrays combined with multiplexed quantitative polymerase chain reactions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::086c282f1ebd371def7227902c8d89bf
http://arxiv.org/abs/1210.1226
http://arxiv.org/abs/1210.1226
Autor:
Laurie Lamoreaux, David Zarkowsky, Richard A. Koup, Mario Roederer, Pratip K. Chattopadhyay, Stephen P. Perfetto
Quantum dots (QD) are fluorescent nanocrystals that are highly useful in imaging and flow cytometric analyses. During routine use of monoclonal antibody conjugates of QD, we have occasionally seen partial or total loss of fluorescence when using cert
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fac9880fcaa2fcdbb329bc39727233b
https://europepmc.org/articles/PMC3073514/
https://europepmc.org/articles/PMC3073514/
Autor:
Laurie Lamoreaux, Richard A. Koup, Stephen P. Perfetto, Pratip K. Chattopadhyay, David R. Ambrozak, Mario Roederer, Richard Nguyen
Publikováno v:
Current Protocols in Cytometry
Amine-reactive dyes, also known as LIVE/DEAD fixable dead cell stains, are a class of viability dyes suitable for identifying dead cells in samples that will be fixed. These dyes cross the cell membranes of dead cells, and react with free amines in t