Zobrazeno 1 - 10
of 105
pro vyhledávání: '"Laurie E. Locascio"'
Publikováno v:
Nature Methods. 11:895-898
‘Irreproducibility’ is symptomatic of a broader challenge in measurement in biomedical research. From the US National Institute of Standards and Technology (NIST) perspective of rigorous metrology, reproducibility is only one aspect of establishi
Publikováno v:
Macromolecules. 45:1602-1611
Quantitative size measurements of single linear and circular DNA molecules in nanofluidic slitlike confinement are reported. A novel experimental method using DNA entropophoresis down a nanofluidic staircase implemented comprehensive variation of sli
Autor:
Samuel M. Stavis, Srinivasa R. Raghavan, Michael Gaitan, Jennifer S. Hong, Silvia H. De Paoli Lacerda, Laurie E. Locascio
Publikováno v:
Langmuir. 26:11581-11588
We present a microfluidic method to direct the self-assembly of temperature-sensitive liposome-hydrogel hybrid nanoparticles. Our approach yields nanoparticles with structural properties and highly monodisperse size distributions precisely controlled
Autor:
Ernest S. Kawasaki, Paul S. Meltzer, Matthew S. Munson, Hope Sedrick, Audrey Player, David Petersen, Jason G. Kralj, Samuel P. Forry, Laurie E. Locascio
Publikováno v:
Lab Chip
We have demonstrated in vitro transcription (IVT) of cDNA sequences from purified Jurkat T-cell mRNA immobilized on microfluidic packed beds down to single-cell quantities. The microfluidically amplified antisense-RNA (aRNA) was nearly identical in l
Publikováno v:
ELECTROPHORESIS. 29:3456-3465
A method is described for an aptamer-based affinity assay using a combination of two nonconventional techniques, temperature gradient focusing (TGF) and field-amplified continuous sample injection TGF (FACSI-TGF), with fluorescence detection. Human i
Autor:
Michael Gaitan, Wyatt N. Vreeland, Srinivasa R. Raghavan, Laurie E. Locascio, Jennifer S. Hong, Silvia H. De Paoli Lacerda
Publikováno v:
Langmuir. 24:4092-4096
Nanosized gel particles (nanogels) are of interest for a variety of applications, including drug delivery and single-molecule encapsulation. Here, we employ the cores of nanoscale liposomes as reaction vessels to template the assembly of calcium algi
Autor:
Andreas Jahn, Joseph E. Reiner, Laurie E. Locascio, Don L. DeVoe, Michael Gaitan, Wyatt N. Vreeland
Publikováno v:
Journal of Nanoparticle Research. 10:925-934
We review a variety of micro- and nanoparticle formulations produced with microfluidic methods. A diverse variety of approaches to generate microscale and nanoscale particles has been reported. Here we emphasize the use of microfluidics, specifically
Publikováno v:
The Journal of Physical Chemistry B. 111:5162-5166
We report on a new method to stabilize nanotube and vesicle structures created from amphiphilic diblock copolymers by means of photopolymerization. Cross-linking with UV light exposure minimizes fluid disruption during stabilization. Additionally, th
Publikováno v:
Journal of AOAC INTERNATIONAL. 90:299-333
The draft ASTM Standard, Standard Practice for Bulk Sample Collection and Swab Sample Collection of Visible Powders Suspected of Being Biological Agents from Nonporous Surfaces,’’ was validated in a collaborative study consisting of 6 teams compr
Autor:
Mulpuri V. Rao, Jayna J. Shah, Michael Gaitan, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland
Publikováno v:
ELECTROPHORESIS. 27:3788-3796
The development of rapid and simple wall coating strategies for high-efficiency electrophoretic separation of DNA is of crucial importance for the successful implementation of miniaturized polymeric DNA analysis systems. In this report, we characteri