Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Laurens Lindenburg"'
Autor:
Laurens Lindenburg, Maarten Merkx
Publikováno v:
Sensors, Vol 14, Iss 7, Pp 11691-11713 (2014)
Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor
Externí odkaz:
https://doaj.org/article/685cedddfaf643f1a4bdf55b2324f699
Autor:
Laurens Lindenburg, Florian Hollfelder
Publikováno v:
Angewandte Chemie. 133:9097-9103
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4908511ea96fe8892e1ffd978102902b
https://doi.org/10.1002/ange.202013486
https://doi.org/10.1002/ange.202013486
Autor:
Eric J. Rees, Jessica A. Downs, Fabrice Gielen, Florian Hollfelder, Maren Butz, Clemens F. Kaminski, Pedro Zuazua-Villar, Marko Hyvönen, Laurens Lindenburg, Teodors Pantelejevs
Publikováno v:
Proc Natl Acad Sci U S A
Exchanges of protein sequence modules support leaps in function unavailable through point mutations during evolution. Here we study the role of the two RAD51-interacting modules within the eight binding BRC repeats of BRCA2. We created 64 chimeric re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d0348d28f1a9ddf322094bf7fdd2fa1
https://doi.org/10.1073/pnas.2017708118
https://doi.org/10.1073/pnas.2017708118
Synthetic DNA is the linchpin of the rapidly accelerating biotechnological era and is perhaps the most promising candidate for long-term digital data storage. Despite huge advances, manufacturing error-free DNA at low cost and high throughput remains
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d87cd678aed892ba8a3aa8b342ebcaa
Publikováno v:
Analytical Chemistry
The precise determination of affinity and specificity is a crucial step in the development of new protein reagents for therapy and diagnostics. Paradoxically, the selection of protein binders, e.g., antibody fragments, from large combinatorial repert
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::296d66d338e5c71e5a88237e6e920b23
https://www.repository.cam.ac.uk/handle/1810/315149
https://www.repository.cam.ac.uk/handle/1810/315149
Autor:
Laurens Lindenburg, Florian Hollfelder
Publikováno v:
Angewandte Chemie (International Ed. in English)
NAD(H)‐utiliing enzymes have been the subject of directed evolution campaigns to improve their function. To enable access to a larger swath of sequence space, we demonstrate the utility of a cell‐free, ultrahigh‐throughput directed evolution pl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2db6df19e9884cbf2179daa0ecc6486e
https://pubmed.ncbi.nlm.nih.gov/33470025
https://pubmed.ncbi.nlm.nih.gov/33470025
Splitmix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins
Autor:
Michael Herger, Michael Snaith, Laurens Lindenburg, Florian Hollfelder, Kayleigh van de Wiel, Tuomas Huovinen
Publikováno v:
Nucleic Acids Research
Site-saturation libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited number of rationally chosen residues. However, uneven library synthesis efficiency leads to amino acid bias, remedied at high cost by e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5ac37768959493ead79a38512ea07a32
https://pubmed.ncbi.nlm.nih.gov/32383757
https://pubmed.ncbi.nlm.nih.gov/32383757
Autor:
M Mantas Malisauskas, LH Laurens Lindenburg, Taf Tari Sips, Maarten Merkx, Sfj van de Graaf, Sjors P. W. Wijnands, Lmpe Lisanne van Oppen
Publikováno v:
Biochemistry, 53, 40, pp. 6370-81
Biochemistry, 53, 6370-81
Biochemistry, 53(40), 6370-6381. American Chemical Society
Biochemistry, 53, 6370-81
Biochemistry, 53(40), 6370-6381. American Chemical Society
Item does not contain fulltext The introduction of weak, hydrophobic interactions between fluorescent protein domains (FPs) can substantially increase the dynamic range (DR) of Forster resonance energy transfer (FRET)-based sensor systems. Here we re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a974642c5dd77f7aacec435f8d5ad4d
https://doi.org/10.1021/bi500433j
https://doi.org/10.1021/bi500433j
Autor:
Laurens Lindenburg, Lovleen Tina Joshi, P. Drevinek, Sarka Vosahlikova, Eshwar Mahenthiralingam, Adam Baldwin, Christopher G. Dowson, Angela Marchbank
Publikováno v:
Journal of Clinical Microbiology. 48:34-40
Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. The clonality of the isolates w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77b4aaac2ba977ed544e737833038d89
https://doi.org/10.1128/jcm.01433-09
https://doi.org/10.1128/jcm.01433-09
Autor:
LH Laurens Lindenburg, Maarten Merkx
Publikováno v:
Sensors, 14(7), 11691-11713. Multidisciplinary Digital Publishing Institute (MDPI)
Sensors, Vol 14, Iss 7, Pp 11691-11713 (2014)
Sensors (Basel, Switzerland)
Sensors
Sensors, Vol 14, Iss 7, Pp 11691-11713 (2014)
Sensors (Basel, Switzerland)
Sensors
Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ff52a6862329384b2373ce2a8637083
https://research.tue.nl/nl/publications/3c90c1dc-e2a6-404d-ba65-9e81c6ef81a2
https://research.tue.nl/nl/publications/3c90c1dc-e2a6-404d-ba65-9e81c6ef81a2