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of 3
pro vyhledávání: '"Laurens H. Lindenburg"'
Autor:
Remkes A. Scheele, Laurens H. Lindenburg, Maya Petek, Markus Schober, Kevin N. Dalby, Florian Hollfelder
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Here, the authors use a droplet-based screen for phosphate transfer catalysis, testing variants of the human protein kinase MKK1 for its ability to activate its downstream target ERK2. Data reveal a flexible motif in the MKK1 docking domain that prom
Externí odkaz:
https://doaj.org/article/5d041683bf7f4218bf7e710dc897fb55
Autor:
Remkes A, Scheele, Laurens H, Lindenburg, Maya, Petek, Markus, Schober, Kevin N, Dalby, Florian, Hollfelder
Publikováno v:
Nature communications. 13(1)
The combination of ultrahigh-throughput screening and sequencing informs on function and intragenic epistasis within combinatorial protein mutant libraries. Establishing a droplet-based, in vitro compartmentalised approach for robust expression and s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cde00523c7ac5e230035aba76a21acdd
https://pubmed.ncbi.nlm.nih.gov/35149678
https://pubmed.ncbi.nlm.nih.gov/35149678
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e82009 (2013)
Magnesium has important structural, catalytic and signaling roles in cells, yet few tools exist to image this metal ion in real time and at subcellular resolution. Here we report the first genetically encoded sensor for Mg(2+), MagFRET-1. This sensor
Externí odkaz:
https://doaj.org/article/aaf52659d13f4a3b9e9670d105d0ff82