Výsledky vyhledávání - "Laurence Hecquet"

Cleavage of Aliphatic α-Hydroxy Ketones by Evolved Transketolase from Geobacillus stearothermophilus

Autor: Hubert Casajus, Aurélie Lagarde, Lionel Nauton, Nazim Ocal, Martin Leremboure, Wolf-Dieter Fessner, Nicolas Duguet, Franck Charmantray, Laurence Hecquet

Publikováno v: ACS Catalysis
ACS Catalysis, 2022, 12, pp.3566-3576. ⟨10.1021/acscatal.1c05140⟩

International audience; The reaction catalyzed by ubiquitous thiamine pyrophosphate-dependent transketolase engaged in cells in the pentose phosphate pathway can be applied in vitro to the cleavage of aliphatic α-hydroxy ketones with thermostable tr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0285802c2de8cc73b082b16aa5d22b4f
https://doi.org/10.1021/acscatal.1c05140

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Co-Immobilization of D-Amino Acid Oxidase, Catalase, and Transketolase for One-Pot, Two-Step Synthesis of L-Erythrulose

Autor: Daria Świętochowska, Aleksandra Łochowicz, Nazim Ocal, Loredano Pollegioni, Franck Charmantray, Laurence Hecquet, Katarzyna Szymańska

Publikováno v: Catalysts
Catalysts, 2023, 13 (1), pp.95. ⟨10.3390/catal13010095⟩
Catalysts; Volume 13; Issue 1; Pages: 95

International audience; Here, we present an immobilized enzyme cascade in a basket-type reactor allowing a one-pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three enzymes, D-amino acid oxidase from Rhodotorula g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e2894d2429a6959839b628ca1cf711c
https://hal.science/hal-03922760

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Transketolase Catalyzed Synthesis of N ‐Aryl Hydroxamic Acids

Autor: Inés Fúster-Fernández, Laurence Hecquet, Wolf-Dieter Fessner

Publikováno v: Advanced Synthesis and Catalysis
Advanced Synthesis and Catalysis, 2022, 364 (3), pp.612-621. ⟨10.1002/adsc.202101100⟩

International audience; Hydroxamic acids are metal-chelating compounds that show important biological activity including anti-tumor effects. We have recently engineered the transketolase from Geobacillus stearothermopilus (TKgst) to convert benzaldeh

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fb1a25c56b72b9a1c2d60369e21a9f0
https://doi.org/10.1002/adsc.202101100

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Synthesis of Deoxysugars from Aliphatic α‐Ketoacids and Aldoses Catalyzed by Thermostable Transketolase Variants from Geobacillus stearothermophilus

Autor: Nazim Ocal, Giuseppe Arbia, Aurélie Lagarde, Muriel Joly, Samantha Gittings, Kirsty M. Graham, Franck Charmantray, Laurence Hecquet

Publikováno v: Advanced Synthesis and Catalysis
Advanced Synthesis and Catalysis, 2022, ⟨10.1002/adsc.202201190⟩

International audience; We described a strategy for the enzymatic synthesis of 1-deoxy and 1,2-deoxyketoses from the aliphatic α-ketoacids, pyruvate and 2-oxobutyrate, as donors and natural aldoses of variable chain length as acceptors, catalyzed by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::de317af02878265368c30ea339ff185a
https://hal.science/hal-03918083/document

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QM/MM Study of Human Transketolase: Thiamine Diphosphate Activation Mechanism and Complete Catalytic Cycle

Autor: Laurence Hecquet, Vincent Théry, Lionel Nauton

Publikováno v: Journal of Chemical Information and Modeling
Journal of Chemical Information and Modeling, American Chemical Society, 2021, ⟨10.1021/acs.jcim.1c00190⟩
Journal of Chemical Information and Modeling, 2021, ⟨10.1021/acs.jcim.1c00190⟩

International audience; A computational model for human transketolase was proposed, showing that thiamine diphosphate activation was based on His110 in place of His481 reported in yeast transketolase. In addition, a complete catalytic reaction pathwa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7df588d053c644a135ba0d688f021d5
https://pubmed.ncbi.nlm.nih.gov/34161071

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Evolved Thermostable Transketolase for Stereoselective Two-Carbon Elongation of Non-Phosphorylated Aldoses to Naturally Rare Ketoses

Autor: Mélanie L’enfant, Romain Dumoulin, Wolf-Dieter Fessner, Franck Charmantray, Agnès Rambourdin, Lionel Nauton, Laurence Hecquet, Marion Lorillière, Vincent Théry

Publikováno v: ACS Catalysis
ACS Catalysis, American Chemical Society, 2019, 9 (6), pp.4754-4763. ⟨10.1021/acscatal.9b01339⟩
ACS Catalysis, 2019, 9 (6), pp.4754-4763. ⟨10.1021/acscatal.9b01339⟩

International audience; We propose an ecofriendly, efficient, stereoselective procedure for the two-carbon elongation of nonphosphorylated aldoses (C4–C6) to the corresponding Cn+2 ketoses (C6–C8) in one step, using hydroxypyruvate (HPA) as a ket

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9db2249d626dd40108f97361b4ede07e
https://doi.org/10.1021/acscatal.9b01339

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High‐Throughput Solid‐Phase Assay for Substrate Profiling and Directed Evolution of Transketolase

Autor: Laurence Hecquet, Nazim Ocal, Aurélie Lagarde, Mélanie L’enfant, Franck Charmantray

Publikováno v: ChemBioChem
ChemBioChem, Wiley-VCH Verlag, In press, ⟨10.1002/cbic.202100356⟩
ChemBioChem, In press, ⟨10.1002/cbic.202100356⟩

International audience; Thiamine diphosphate-dependent enzymes, and specifically transketolases, form one of the most important families of biocatalytic tools for enantioselective carbon-carbon bond formation yielding various hydroxyketones of biolog

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8d6ad3900d61a8def8fca0beebfdd63
https://hal.archives-ouvertes.fr/hal-03329188

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d -Serine as a Key Building Block: Enzymatic Process Development and Smart Applications within the Cascade Enzymatic Concept

Autor: Franck Charmantray, Nazim Ocal, Laurence Hecquet, Loredano Pollegioni, Jérôme Collin, Pascal Auffray, Juliette Martin, Mélanie L’enfant

Publikováno v: Organic Process Research and Development
Organic Process Research and Development, American Chemical Society, 2020, 24 (5), pp.769-775. ⟨10.1021/acs.oprd.0c00024⟩
Organic Process Research and Development, 2020, 24 (5), pp.769-775. ⟨10.1021/acs.oprd.0c00024⟩

International audience; An efficient enzymatic method catalyzed by an enzyme from the d-threonine aldolase (DTA) family was developed for d-serine production at industrial scale. This process was used for the synthesis of two valuable ketoses, l-eryt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1a007f821f0e46cb08c437756212e8a
https://hal.archives-ouvertes.fr/hal-02907525

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Convergent in situ Generation of Both Transketolase Substrates via Transaminase and Aldolase Reactions for Sequential One‐Pot, Three‐Step Cascade Synthesis of Ketoses

Autor: Marion Lorillière, Laurence Hecquet, Wolf-Dieter Fessner, Pere Clapés, Christine Guérard-Hélaine, Franck Charmantray, Thierry Gefflaut

Publikováno v: ChemCatChem
ChemCatChem, Wiley, 2020, 12 (3), pp.812-817. ⟨10.1002/cctc.201901756⟩
ChemCatChem, 2020, 12 (3), pp.812-817. ⟨10.1002/cctc.201901756⟩
Digital.CSIC. Repositorio Institucional del CSIC
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International audience; We describe an efficient three‐enzyme, sequential one‐pot cascade reaction where both transketolase substrates are generated in situ in a convergent fashion. The nucleophilic donor substrate hydroxypyruvate was obtained fr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::937909a3b48328dbabf3143197c8921e
https://hal.archives-ouvertes.fr/hal-02991624

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