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Autor:
Sarah A. Sacco, Angela M. Tuckowski, Irina Trenary, Lauren Kraft, Michael J. Betenbaugh, Jamey D. Young, Kevin D. Smith
Publikováno v:
Biotechnology and Bioengineering. 119:1712-1727
The glutamine synthetase (GS) expression system is commonly used to ensure stable transgene integration and amplification in Chinese hamster ovary (CHO) host lines. Transfected cell populations are typically grown in the presence of the GS inhibitor,