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pro vyhledávání: '"Laureen M.Y. Mertens"'
Autor:
Elisa Consoli, Tanneke den Blaauwen, Anna O. Chertkova, Nils Y. Meiresonne, Laureen M.Y. Mertens, Joachim Goedhart
Publikováno v:
Molecular Microbiology
Molecular Microbiology, 111(4), 1025-1038. Wiley-Blackwell
Molecular Microbiology, 111(4), 1025-1038. Wiley-Blackwell
Summary Fluorescent proteins (FPs) are of vital importance to biomedical research. Many of the currently available fluorescent proteins do not fluoresce when expressed in non‐native environments, such as the bacterial periplasm. This strongly limit
Publikováno v:
BIO-PROTOCOL. 9(23)
This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in all compartments of Escherichia coli by Forster Resonance Energy Transfer (FRET) using the Superfolder mTurquoise2 ox-mNeonGreen FRET pair (sfTq2ox