Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Laura van Weeren"'
Autor:
Joachim Goedhart, Laura van Weeren, Merel J W Adjobo-Hermans, Ies Elzenaar, Mark A Hink, Theodorus W J Gadella
Publikováno v:
PLoS ONE, Vol 6, Iss 11, p e27321 (2011)
BACKGROUND: Co-expression of proteins is generally achieved by introducing two (or more) independent plasmids into cells, each driving the expression of a different protein of interest. However, the relative expression levels may vary strongly betwee
Externí odkaz:
https://doaj.org/article/b4af9cd9a1fb4c00a7d31d143907ddba
Autor:
Joachim Goedhart, Joop E M Vermeer, Merel J W Adjobo-Hermans, Laura van Weeren, Theodorus W J Gadella
Publikováno v:
PLoS ONE, Vol 2, Iss 10, p e1011 (2007)
BACKGROUND: Fluorescence Resonance Energy Transfer (FRET) between the green fluorescent protein (GFP) variants CFP and YFP is widely used for the detection of protein-protein interactions. Nowadays, several monomeric red-shifted fluorescent proteins
Externí odkaz:
https://doaj.org/article/2ea7e26f91614f0f8caa0c0f0a3b2314
Autor:
Theodorus W. J. Gadella, Laura van Weeren, Jente Stouthamer, Mark A. Hink, Anouk H. G. Wolters, Ben N. G. Giepmans, Sylvain Aumonier, Jérôme Dupuy, Antoine Royant
Publikováno v:
Nature Methods, 20(4), 541-545. Nature Publishing Group
Nature Methods
Nature Methods, 2023, 20 (4), pp.541-545. ⟨10.1038/s41592-023-01809-y⟩
Nature Methods
Nature Methods, 2023, 20 (4), pp.541-545. ⟨10.1038/s41592-023-01809-y⟩
We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a
Autor:
Laura van Weeren, Christian Blum, Jente Stouthamer, Daphne S. Bindels, Vinod Subramaniam, Theodorus W. J. Gadella, Jord C. Prangsma, Robert Molenaar, Lindsay Haarbosch, Willem L. Vos
Publikováno v:
The journal of Physical Chemistry. B, 124(8), 1383-1391. American Chemical Society
The Journal of physical chemistry B, 124(8), 1383-1391. American Chemical Society
The Journal of Physical Chemistry. B
The Journal of physical chemistry B, 124(8), 1383-1391. American Chemical Society
The Journal of Physical Chemistry. B
The fluorescence quantum yield of four representative red fluorescent proteins mCherry, mKate2, mRuby2, and the recently introduced mScarlet was investigated. The excited state lifetimes were measured as a function of the distance to a gold mirror in
Autor:
Lindsay Haarbosch, Daphne S. Bindels, Marten Postma, Laura van Weeren, Theodorus W. J. Gadella
Publikováno v:
Nature Protocols, 15(2), 450-478. Nature Publishing Group
Genetically encoded fluorescent proteins (FPs) are highly utilized in cell biology research to study proteins of interest or signal processes using biosensors. To perform well in specific applications, these FPs require a multitude of tailored proper
Publikováno v:
American Journal of Physiology-Gastrointestinal and Liver Physiology. 293:G165-G177
Rab3D is a small GTP-binding protein that associates with secretory granules of endocrine and exocrine cells. The physiological role of Rab3D remains unclear. While it has initially been implicated in the control of regulated exocytosis, recent delet
Autor:
Mark A. Hink, Kees Jalink, Norbert O. E. Vischer, Laura van Weeren, Joachim Goedhart, Theodorus W. J. Gadella
Publikováno v:
Nature Methods, 7(2), 137-139. Nature Publishing Group
Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins w
Autor:
Daphne S, Bindels, Joachim, Goedhart, Mark A, Hink, Laura, van Weeren, Linda, Joosen, Theodorus W J, Gadella
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1076
Nowadays, fluorescent protein (FP) variants have been engineered to fluoresce in all different colors; to display photoswitchable, or photochromic, behavior; or to show yet other beneficial properties that enable or enhance a still growing set of new
Autor:
Laura van Weeren, Merel J. W. Adjobo-Hermans, Joachim Goedhart, Kevin C. Crosby, Carsten Schultz, Mateusz Putyrski, Theodorus W. J. Gadella, Arshia Bhageloe
Publikováno v:
Cellular Signalling, 25, 255-63
Cellular Signalling, 25, 1, pp. 255-63
Cellular Signalling, 25(1), 255-263. Elsevier
Cellular Signalling, 25, 1, pp. 255-63
Cellular Signalling, 25(1), 255-263. Elsevier
Item does not contain fulltext Phospholipase C (PLC) beta isoforms are implicated in various physiological processes and pathologies. However, mechanistic insight into the localization and activation of each of the isoforms is limited. Therefore, it
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c32ceebca1576b63c516f4b237d05eb
http://hdl.handle.net/2066/118918
http://hdl.handle.net/2066/118918