In vitro Antigen-presentation Assay for Self- and Microbial-derived Antigens

Autor: Laura Campisi

Publikováno v: Bio-Protocol, Vol 7, Iss 11 (2017)

Antigen presenting cells (APC) are able to process and present to T cells antigens from different origins. This mechanism is highly regulated, in particular by Patter Recognition Receptor (PRR) signals. Here, I detail a protocol designed to assess in

Externí odkaz: https://doaj.org/article/3f783fc983df47d7bef8ce9dffa7906d

Clonally expanded CD8 T cells characterize Amyotrophic Lateral Sclerosis 4

Autor: Cindy Beharry, Jessica Sook Yuin Ho, Frederick Arnold, Lorena Mosca, Anastasia Gromova, Lyle Ostrow, Laura Campisi, Ivan Marazzi, Neil A. Shneider, Shahab Chizari, Xueyan Mei, Yesai Fstkchyan, Ning Jiang, Christian Lunetta, Albert R. La Spada, Jack Prazich, Craig L. Bennett, Vlad Korobeynikov

Amyotrophic Lateral Sclerosis (ALS) is a heterogeneous neurodegenerative disorder that affects motor neurons in the brain and spinal cord, causing progressive loss of voluntary muscle control1,2. ALS heterogeneity includes the age of manifestation, t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a987560b57d8d93473b77f33b2a31d2c
https://doi.org/10.21203/rs.3.rs-798338/v1

TOP1 inhibition therapy protects against SARS-CoV-2-induced lethal inflammation

Autor: Jessie D. Trujillo, Emily R. Miraldi, Jan Bakker, Benjamin R. tenOever, Andre L. Moreira, Helen Ray-Jones, Ivan Marazzi, Simin Zheng, Nan Zhao, Laura Campisi, Michael Schotsaert, Zeyu Zhu, Sven Heinz, Elaine Shum, Christopher Benner, Jessica Sook Yuin Ho, Anna Junxia Zhang, Adolfo García-Sastre, Sreeja Parameswaran, Joseph A. Wayman, Matthew T. Weirauch, Anand D. Jeyasekharan, Yesai Fstkchyan, Igor Morozov, Wen-Chun Liu, Minji Byun, Sumit K. Chanda, Sabarish V. Indran, Natasha N. Gaudreault, Mikhail Spivakov, Honglin Chen, Alba Escalera, Bobo Wing-Yee Mok, Kris M. White, Juergen A. Richt, Mariano Carossino, Valeriya Malysheva, Randy A. Albrecht, Tristan X. Jordan, Teresa Aydillo, Robert Sebra, Betsaida Salom Melo, Andrew Chak-Yiu Lee, Sonia Jangra, Udeni B. R. Balasuriya, David A. Meekins, Ernesto Guccione, Siu Ying Lau, Soner Yildiz, Michiel J. Thiecke, David A. Kaufman, Raveen Rathnasinghe, Honglian Liu

Publikováno v: Cell, 184(10), 2618-2632.e17. Cell Press
Cell, vol 184, iss 10
Cell

The ongoing pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is currently affecting millions of lives worldwide. Large retrospective studies indicate that an elevated level of inflammatory cytokines and pro-inflammatory

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d169781a64bf58af62230f5b19d8e6ad
https://pure.eur.nl/en/publications/c6a3d84a-3a2d-4ff6-9904-1d7f6b018446

Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection

Autor: Jiajie Wei, Paul Digard, Robert J. Gifford, Nerea Irigoyen, Megan K. L. MacLeod, Alessandro Vannini, James P. Gibbs, Laura Campisi, Brad R. Rosenberg, Joshua D. Jones, Max W. Chang, Cheng Huang, Quan Gu, Edward C. Hutchinson, Veronica V. Rezelj, Christopher Benner, Helen M. Wise, Jessica Sook Yuin Ho, Jeffrey R. Johnson, Guojun Wang, Matthew Angel, Yesai Fstkchyan, Slobodan Paessler, Robyn M. Kaake, Nan Zhao, Maria João Amorim, Marta Alenquer, Elizabeth Sloan, Sara Clohisey, Ingeborg van Knippenberg, Harm van Bakel, Simin Zheng, Nevan J. Krogan, Liliane Chung, Adam M. Dinan, Bo Wang, Benjamin Greenbaum, Léa Meyer, Natasha Moshkina, Ian Brierley, Zeyu Zhu, Zuleyma Peralta, Adolfo García-Sastre, Andrew E. Firth, Marta Łuksza, Emily R. Miraldi, Vladimir Roudko, Ivan Marazzi, Rong Shen, Carles Martínez-Romero, Yixuan Ma, Jonathan W. Yewdell, J Kenneth Baillie, Justine Noel

Publikováno v: Yuin Ho, J S, Angel, M, Ma, Y, Sloan, E, Wang, G, Martinez-Romero, C, Alenquer, M, Roudko, V, Chung, L, Zheng, S, Chang, M, Fstkchyan, Y, Clohisey, S, Dinan, A M, Gibbs, J, Gifford, R, Shen, R, Gu, Q, Irigoyen, N, Campisi, L, Huang, C, Zhao, N, Jones, J, van Knippenberg, I, Zhu, Z, Moshkina, N, Meyer, L, Noel, J, Peralta, Z, Rezelji, V, Kaake, R, Rosenberg, B, Wang, B, Wei, J, Paessler, S, Wise, H, Johnson, J, Vannini, A, Amorim, M J, Baillie, K, Miraldi, E R, Benner, C, Brierley, I, Digard, P, Luksza, M, Firth, A E, Krogan, N J, Greenbaum, B D, MacLeod, M K, van Bakel, H, García-Sastre, A, Yewdell, J W, Hutchinson, E & Marrazi, I 2020, ' Hybrid Gene Origination Creates Human-Virus Chimeric Protein During Infection ', Cell, vol. 181, no. 7, pp. 1502-1517 . https://doi.org/10.1016/j.cell.2020.05.035
Cell

RNA viruses are a major human health threat. The life cycles of many highly pathogenic RNA viruses like influenza A virus (IAV) and Lassa virus depends on host mRNA, as viral polymerases cleave 5’-m7G-capped host transcripts to primeviral mRNA synt

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63665551fab0a3b9b7b770a82f00e7e2
https://www.repository.cam.ac.uk/handle/1810/307677

A CD8 T cell signature in ALS4

Autor: Laura Campisi, Anastasia Gromova, Frederick Arnold, Xueyan Mei, Cindy Beharry, Yesai Fstkchyan, Albert La Spada, Ivan Marazzi

Publikováno v: The Journal of Immunology. 206:12.06-12.06

Motor neuron disorders (MND) cause disability and death globally. Despite an increasing knowledge of the genetic defects of MND, a clear understanding of the mechanisms of disease initiation and progression is lacking. Recent advance in the field has

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0e5eb69a4a136d1c300998dc8f401b41
https://doi.org/10.4049/jimmunol.206.supp.12.06