Building a super-resolution fluorescence cryomicroscope.

Autor: Last MGF; Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands., Voortman LM; Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands., Sharp TH; Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands; School of Biochemistry, University of Bristol, Bristol, United Kingdom. Electronic address: t.sharp@bristol.ac.uk.

Publikováno v: Methods in cell biology [Methods Cell Biol] 2024; Vol. 187, pp. 205-222. Date of Electronic Publication: 2024 Mar 13.

Super-resolution fluorescence imaging of cryosamples does not limit achievable resolution in cryoEM.

Autor: Last MGF; Department of Cell and Chemical Biology, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands., Noteborn WEM; Netherlands Centre for Electron Nanoscopy, Leiden University, 2333 AL Leiden, The Netherlands., Voortman LM; Department of Cell and Chemical Biology, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands., Sharp TH; Department of Cell and Chemical Biology, Leiden University Medical Centre, 2300 RC Leiden, The Netherlands. Electronic address: t.sharp@lumc.nl.

Publikováno v: Journal of structural biology [J Struct Biol] 2023 Dec; Vol. 215 (4), pp. 108040. Date of Electronic Publication: 2023 Oct 31.

Measuring cryo-TEM sample thickness using reflected light microscopy and machine learning.

Autor: Last MGF; Department of Cell and Chemical Biology, Leiden University Medical Center, 2300 RC Leiden, the Netherlands., Voortman LM; Department of Cell and Chemical Biology, Leiden University Medical Center, 2300 RC Leiden, the Netherlands., Sharp TH; Department of Cell and Chemical Biology, Leiden University Medical Center, 2300 RC Leiden, the Netherlands. Electronic address: t.sharp@lumc.nl.

Publikováno v: Journal of structural biology [J Struct Biol] 2023 Jun; Vol. 215 (2), pp. 107965. Date of Electronic Publication: 2023 Apr 24.

A cryogenic, coincident fluorescence, electron, and ion beam microscope.

Autor: Boltje DB; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands.; Delmic B.V, Delft, Netherlands., Hoogenboom JP; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Jakobi AJ; Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands., Jensen GJ; California Institute of Technology, Pasadena, United States.; Brigham Young University, Provo, United States., Jonker CTH; Delmic B.V, Delft, Netherlands., Kaag MJ; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Koster AJ; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, Netherlands., Last MGF; Delmic B.V, Delft, Netherlands.; Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, Netherlands., de Agrela Pinto C; Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands., Plitzko JM; CryoEM Technology, Max Planck Institute of Biochemistry, Martinsried, Germany., Raunser S; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., Tacke S; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., Wang Z; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund, Germany., van der Wee EB; Department of Imaging Physic, Delft University of Technology, Delft, Netherlands., Wepf R; Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Australia., den Hoedt S; Delmic B.V, Delft, Netherlands.

Publikováno v: ELife [Elife] 2022 Oct 28; Vol. 11. Date of Electronic Publication: 2022 Oct 28.

Spatiotemporal control of coacervate formation within liposomes.

Autor: Deshpande S; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Brandenburg F; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Lau A; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Last MGF; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Spoelstra WK; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Reese L; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Wunnava S; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Dogterom M; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands., Dekker C; Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands. c.dekker@tudelft.nl.

Publikováno v: Nature communications [Nat Commun] 2019 Apr 17; Vol. 10 (1), pp. 1800. Date of Electronic Publication: 2019 Apr 17.