Zobrazeno 1 - 10
of 320
pro vyhledávání: '"Lars K, Nielsen"'
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 4, Pp 101329- (2024)
Producing recombinant adeno-associated virus (rAAV) for gene therapy via triple transfection is an intricate process involving many cellular interactions. Each of the different elements encoded in the three required plasmids—pHelper, pRepCap, and p
Externí odkaz:
https://doaj.org/article/1178c71431a243ac95027659e4a9f5b6
Publikováno v:
BMC Bioinformatics, Vol 25, Iss 1, Pp 1-8 (2024)
Abstract Background Uniform random sampling of mass-balanced flux solutions offers an unbiased appraisal of the capabilities of metabolic networks. Unfortunately, it is impossible to avoid thermodynamically infeasible loops in flux samples when using
Externí odkaz:
https://doaj.org/article/a4a51a71659541a9b4a9a796cf63dcea
Autor:
Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, Kedar N. Natarajan, Lars K. Nielsen, Tanya Warnecke, Ryan T. Gill
Publikováno v:
iScience, Vol 26, Iss 12, Pp 108287- (2023)
Summary: Discovery of genomic safe harbor sites (SHSs) is fundamental for multiple transgene integrations, such as reporter genes, chimeric antigen receptors (CARs), and safety switches, which are required for safe cell products for regenerative cell
Externí odkaz:
https://doaj.org/article/1ed7e3c6b32b4486a833882c76203d11
Publikováno v:
Journal of Translational Medicine, Vol 20, Iss 1, Pp 1-13 (2022)
Abstract The human microbiome has been linked to several diseases. Gastrointestinal diseases are still one of the most prominent area of study in host-microbiome interactions however the underlying microbial mechanisms in these disorders are not full
Externí odkaz:
https://doaj.org/article/62bc145858fb48528db341b6a39bab05
Autor:
Michael A. MacDonald, Matthias Nöbel, Verónica S. Martínez, Kym Baker, Evan Shave, Peter P. Gray, Stephen Mahler, Trent Munro, Lars K. Nielsen, Esteban Marcellin
Publikováno v:
mAbs, Vol 14, Iss 1 (2022)
The reliable and cost-efficient manufacturing of monoclonal antibodies (mAbs) is essential to fulfil their ever-growing demand. Cell death in bioreactors reduces productivity and product quality, and is largely attributed to apoptosis. In perfusion b
Externí odkaz:
https://doaj.org/article/652962a831424760825d57ea8ed6a53f
Publikováno v:
Bioengineering, Vol 10, Iss 5, p 576 (2023)
Genome-scale metabolic models (GEMs) have emerged as a tool to understand human metabolism from a holistic perspective with high relevance in the study of many diseases and in the metabolic engineering of human cell lines. GEM building relies on eith
Externí odkaz:
https://doaj.org/article/8490cb9f5df74963ace7e37325abd91c
Autor:
Verónica S Martínez, Pedro A Saa, Jason Jooste, Kanupriya Tiwari, Lake-Ee Quek, Lars K Nielsen
Publikováno v:
PLoS Computational Biology, Vol 18, Iss 6, p e1010203 (2022)
The topology of metabolic networks is recognisably modular with modules weakly connected apart from sharing a pool of currency metabolites. Here, we defined modules as sets of reversible reactions isolated from the rest of metabolism by irreversible
Externí odkaz:
https://doaj.org/article/8ee6253ece2b44098f1e3712b65e40d6
Publikováno v:
Proteomes, Vol 11, Iss 2, p 11 (2023)
Although numerous studies support a dose–effect relationship between Endocrine disruptors (EDs) and the progression and malignancy of tumors, the impact of a chronic exposure to non-lethal concentrations of EDs in cancer remains unknown. More speci
Externí odkaz:
https://doaj.org/article/6d93751f1a3f4ba3be4fdc83b860f54c
Autor:
Igor Marín de Mas, Laura Torrents, Carmen Bedia, Lars K. Nielsen, Marta Cascante, Romà Tauler
Publikováno v:
BMC Genomics, Vol 20, Iss 1, Pp 1-12 (2019)
Abstract Background Genome-scale metabolic models (GSMM) integrating transcriptomics have been widely used to study cancer metabolism. This integration is achieved through logical rules that describe the association between genes, proteins, and react
Externí odkaz:
https://doaj.org/article/48694ea0f65d4187b23963ce61975b0f
Autor:
Jessica L Schwaber, Darren Korbie, Stacey Andersen, Erica Lin, Panagiotis K Chrysanthopoulos, Matt Trau, Lars K Nielsen
Publikováno v:
PLoS ONE, Vol 16, Iss 2, p e0246107 (2021)
With the exception of a few master transcription factors, regulators of neutrophil maturation are poorly annotated in the intermediate phenotypes between the granulocyte-macrophage progenitor (GMP) and the mature neutrophil phenotype. Additional chal
Externí odkaz:
https://doaj.org/article/e3c9c0d3cbf44e50a004e39fe0f42450