Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Larry J, Cummings"'
Autor:
Larry J, Cummings
Publikováno v:
Methods in enzymology. 541
The purpose of this section is to provide developmental purification strategies for recombinant proteins on a column of hydroxyapatite and then to optimize them for repeated purification cycles using effect additives. Surface neutralization solutions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7d97775aee84d47c7e84672976093e7f
https://pubmed.ncbi.nlm.nih.gov/24674063
https://pubmed.ncbi.nlm.nih.gov/24674063
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1131
Hydroxyapatite chromatography is shown to be an excellent method for chromatographically purifying monoclonal antibodies (Mab). Mab contained in eluates from Protein A columns was partially purified on ceramic hydroxyapatite (CHT™) Type I, 40 μm c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dcec364e5ea01346b837161eecf3557f
https://pubmed.ncbi.nlm.nih.gov/24515470
https://pubmed.ncbi.nlm.nih.gov/24515470
Autor:
Larry J. Cummings
The purpose of this section is to provide developmental purification strategies for recombinant proteins on a column of hydroxyapatite and then to optimize them for repeated purification cycles using effect additives. Surface neutralization solutions
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ff1b2a6619bece6f8d0a795769785e90
https://doi.org/10.1016/b978-0-12-420119-4.00006-9
https://doi.org/10.1016/b978-0-12-420119-4.00006-9
Publikováno v:
Methods in Molecular Biology ISBN: 9781627039918
Hydroxyapatite chromatography is shown to be an excellent method for chromatographically purifying monoclonal antibodies (Mab). Mab contained in eluates from Protein A columns was partially purified on ceramic hydroxyapatite (CHT™) Type I, 40 μm c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c15279909850cd92b393c91e8aafc64a
https://doi.org/10.1007/978-1-62703-992-5_15
https://doi.org/10.1007/978-1-62703-992-5_15
Autor:
Larry J. Cummings, Mark A. Snyder, Nick Keener, Brian Williamson, Shawn Cao, Oliver Kaltenbrunner, Nancy Jiao, Esteban Freydell, Li Zhu
Publikováno v:
Biotechnology journal. 7(10)
Ceramic hydroxyapatite (CHT) is a multimodal chromatographic medium widely used in the pharmaceutical industry for the purification of biomolecules. CHT is a sintered form of hydroxyapatite crystals with moderate stability at acidic conditions. This
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef1652f756f7ca474b789f0c4faf7bcb
https://pubmed.ncbi.nlm.nih.gov/22674902
https://pubmed.ncbi.nlm.nih.gov/22674902
Publikováno v:
Methods in enzymology. 463
The introduction of spherical forms of hydroxyapatite has enabled protein scientists to separate and purify proteins multiple times with the same packed column. Biopharmaceutical companies have driven single column applications of complex samples to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1ca8e09263d5f0a03918ad3e7fc8209e
https://pubmed.ncbi.nlm.nih.gov/19892184
https://pubmed.ncbi.nlm.nih.gov/19892184
Characterization of synthetic macroporous ion-exchange resins in low-pressure cartridges and columns
Autor:
Christopher J. Siebert, Lee C. Dunn, Larry J. Cummings, Mohsen Navvab, Cheryl Ordunez, Kenneth W. Talmadge, Mohamed Aboulezz
Publikováno v:
Journal of Chromatography A. 548:165-178
Three ion-exchange materials and one hydrophobic-interaction chromatography packing, based on a rigid macroporous polymer with large, relatively uniform pores, have been evaluated for low-pressure liquid chromatography of antibodies. These sorbents h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f649612a2c720289a7de66763065ba36
https://doi.org/10.1016/s0021-9673(01)88599-x
https://doi.org/10.1016/s0021-9673(01)88599-x
Autor:
Leroy B. Beasley, Larry J. Cummings
Publikováno v:
Linear and Multilinear Algebra. 5:297-302
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a6d489d1c558a699aa76b59271d4f14e
https://doi.org/10.1080/03081087808817210
https://doi.org/10.1080/03081087808817210
Autor:
Lee C. Dunn, Christopher J. Siebert, Larry J. Cummings, Gary S. Ott, J.Keith Duncan, David J. Burke
Publikováno v:
Journal of Chromatography A. 353:425-437
A new anion-exchange material has been developed which allows very rapid resolution of protein mixtures. The Microanalyzer MA7P matrix consists of small (7 micron), spherical, non-porous, polymethacrylate beads with polyethyleneimine (PEI) covalently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26d3f3faa7a67865c90b5e2b8f8f8c54
https://doi.org/10.1016/s0021-9673(01)87113-2
https://doi.org/10.1016/s0021-9673(01)87113-2
Publikováno v:
Journal of Chromatographic Science. 18:551-558
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::846545f5cf605d5fae2bcf4627824555
https://doi.org/10.1093/chromsci/18.10.551
https://doi.org/10.1093/chromsci/18.10.551