Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Larry A. Gilbertson"'
Autor:
Xudong Ye, Zarir Vaghchhipawala, Edwards J. Williams, Changlin Fu, Jinyuan Liu, Fengming Lu, Erin L. Hall, Shirley X. Guo, LaRee Frank, Larry A. Gilbertson
Publikováno v:
Plant Cell Reports. 42:45-55
Selectable marker genes are often required for efficient generation of transgenic plants in tissue culture transformation systems but are not desired once the transgenic events are obtained. We have developed Cre/loxP autoexcision systems to remove s
Autor:
Zarir Vaghchhipawala, Sharon Radke, Ervin Nagy, Mary L Russell, Susan Johnson, Stanton B Gelvin, Larry A Gilbertson, Xudong Ye
Publikováno v:
PLoS ONE, Vol 13, Iss 11, p e0200972 (2018)
A native repABC replication origin from pRiA4b was previously reported as a single copy plasmid in Agrobacterium tumefaciens and can improve the production of transgenic plants with a single copy insertion of transgenes when it is used in binary vect
Externí odkaz:
https://doaj.org/article/f89990d74f72413389eeac8aa3592d60
Autor:
Ervin D. Nagy, Rosemarie Kuehn, Dafu Wang, Ashok Shrawat, David M. Duda, Jeanna R. Groat, Peizhen Yang, Steven Beach, Yuanji Zhang, Linda Rymarquis, Sharina L. Carter, Robert T. Gaeta, Larry A. Gilbertson
Publikováno v:
The Plant journal : for cell and molecular biologyREFERENCES. 111(3)
High efficiency site-directed chromosomal integration of exogenous DNA in plants remains a challenge despite recent advances in genome editing technologies. One approach to mitigate this problem is to increase the effective concentration of the donor
Publikováno v:
Plant Cell Reports. 35:601-611
KEY MESSAGE : virG mutant strains of a nopaline type of Agrobacterium tumefaciens increase the transformation frequency in cotton meristem transformation. Constitutive cytokinin expression from the tzs gene in the virG mutant strains is responsible f
Autor:
Mary L. Russell, Larry A. Gilbertson, Stanton B. Gelvin, Vaghchhipawala Zarir, Ervin D. Nagy, Susan Johnson, Xudong Ye, Sharon Radke
A nativerepABCreplication origin, ori pRi, was previously reported as a single copy plasmid inAgrobacterium tumefaciensand can improve the production of transgenic plants with a single copy insertion of transgenes when it is used in binary vectors fo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95e06591d711b6e548c03d7a5d4d39c1
https://doi.org/10.1101/365320
https://doi.org/10.1101/365320
Autor:
Larry A. Gilbertson, Zarir Vaghchhipawala, Ervin D. Nagy, Sharon Radke, Mary L. Russell, Stanton B. Gelvin, Xudong Ye, Susan Johnson
Publikováno v:
PLoS ONE
PLoS ONE, Vol 13, Iss 11, p e0200972 (2018)
PLoS ONE, Vol 13, Iss 11, p e0200972 (2018)
A native repABC replication origin from pRiA4b was previously reported as a single copy plasmid in Agrobacterium tumefaciens and can improve the production of transgenic plants with a single copy insertion of transgenes when it is used in binary vect
Autor:
Brenda Lowe, Michael W. Petersen, Junjiang Shen, Larry A. Gilbertson, Steve Strickland, Susan Johnson, James A. Esser, Sharon Radke, Xudong Ye, Edward J. Williams
Publikováno v:
Transgenic Research. 20:773-786
Single transgene copy, vector backbone-free transgenic crop plants are highly desired for functional genomics and many biotechnological applications. We demonstrate that binary vectors that use a replication origin derived from the Ri plasmid of Agro
Autor:
Xudong Ye, James A. Esser, Junjiang Shen, Larry A. Gilbertson, Amy M. Nichols, Edward J. Williams, Michael W. Petersen
Publikováno v:
Transgenic Research. 17:827-838
Conventional Agrobacterium-mediated plant transformation often produces a significant frequency of transgenic events containing vector backbone sequence, which is generally undesirable for biotechnology applications. We tested methods to reduce the f
Publikováno v:
Plant Cell Reports. 25:320-328
Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l(-1) picloram and 3 mg l(-1) 6-benzylaminopurine, which i
Autor:
Larry A. Gilbertson
Publikováno v:
Trends in Biotechnology. 21:550-555
Targeted insertion and the precise deletion of DNA from transgenic plant chromosomes increase the potential of plant biotechnology for commercial applications and basic research. The Cre– lox recombination system is one of the best characterized an