Comparison of different methods to overexpress large genes

Autor: Aline Lisie Ramos, Fernanda Soares Niemann, Adriana Silva Santos Duarte, Karla Priscila Ferro, Irene Santos, Carolina Louzão Bigarella, Antonio Filareto, Sara Teresinha Olalla Saad

Publikováno v: Journal of Biological Research, Vol 91, Iss 2 (2018)

Gain-of-function of very large transgene constructs can lead to genetic perturbations, providing researchers with the alternative of a powerful tool to identify pathway components which remain undetected when using traditional loss-of-function analys

Externí odkaz: https://doaj.org/article/b0f5c182e7f045ddab94e7796d36e1e0

Large Intronic Deletion of the Fragile Site Gene PRKN Dramatically Lowers Its Fragility Without Impacting Gene Expression

Autor: Vasileios Voutsinos, Sebastian H. N. Munk, Vibe H. Oestergaard

Publikováno v: Frontiers in Genetics, Vol 12 (2021)
Munk, S H N, Voutsinos, V & Oestergaard, V H 2021, ' Large Intronic Deletion of the Fragile Site Gene PRKN Dramatically Lowers Its Fragility Without Impacting Gene Expression ', Frontiers in Genetics, vol. 12, 695172 . https://doi.org/10.3389/fgene.2021.695172

Common chromosomal fragile sites (CFSs) are genomic regions prone to form breaks and gaps on metaphase chromosomes during conditions of replication stress. Moreover, CFSs are hotspots for deletions and amplifications in cancer genomes. Fragility at C

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a30362db497bbce0ec6980df1693836
https://www.frontiersin.org/articles/10.3389/fgene.2021.695172/full

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Common Chromosomal Fragile Sites—Conserved Failure Stories

Autor: Vibe H. Oestergaard, Sebastian H. N. Munk, Vasileios Voutsinos

Publikováno v: Voutsinos, V, Munk, S H N & Østergaard, V H 2018, ' Common Chromosomal Fragile Sites-Conserved Failure Stories ', Genes, vol. 9, no. 12, 580, pp. 1-16 . https://doi.org/10.3390/genes9120580
Genes
Genes, Vol 9, Iss 12, p 580 (2018)

In order to pass on an intact copy of the genome during cell division, complete and faithful DNA replication is crucial. Yet, certain areas of the genome are intrinsically challenging to replicate, which manifests as high local mutation propensity. S

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29fe3bb5f405bedddc2f3de411bb4942
https://doi.org/10.3390/genes9120580

The Drosophila homologue of the dystrophin gene - introns containing promoters are the major contributors to the large size of the gene

Autor: David Yaffe, Moran Kovalio, Uri Nudel, Sara Neuman

Publikováno v: FEBS letters. 579(24)

We show that the drosophila gene encoding the dystrophin-like protein (DLP) is as complex as the mammalian dystrophin gene. Three 5′ promoters and three internal promoters regulate the expression of three full-length and three truncated products, r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10aaae0cef99066d518b0a5321d92db6
https://pubmed.ncbi.nlm.nih.gov/16198353