Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Lance Koutny"'
Publikováno v:
ELECTROPHORESIS. 22:4104-4117
A number of significant improvements in the electrophoretic performance and design of DNA sequencing devices have culminated in the introduction of truly industrial grade production scale instruments. These instruments have been the workhorses behind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbe173383943fb5d58fd53d9639df14e
https://doi.org/10.1002/1522-2683(200111)22:19<4104::aid-elps4104>3.0.co
https://doi.org/10.1002/1522-2683(200111)22:19<4104::aid-elps4104>3.0.co
Autor:
Scott Buonocore, Aram Adourian, Oscar Salas-Solano, Dieter Schmalzing, Sameh El-Difrawy, Lance Koutny, Paul A. McEwan, Daniel J. Ehrlich, Kevin Abbey, and Paul Matsudaira
Publikováno v:
Analytical Chemistry. 72:3388-3391
The human genome will be sequenced using capillary array electrophoresis technology. Although currently achieving only 550 base reads per run, capillary arrays have increased the efficiency and lowered the cost of sequencing by eliminating gel plate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee269914bcb9c78be4c1d46168112506
https://doi.org/10.1021/ac9913614
https://doi.org/10.1021/ac9913614
Autor:
Lance Koutny, Aram Adourian, Oscar Salas-Solano, Daniel J. Ehrlich, Scott Buonocore, Paul Matsudaira, Dieter Schmalzing
Publikováno v:
Analytical Chemistry. 72:3129-3137
We have examined the parametric performance of short microfabricated electrophoresis devices that operate with a replaceable linear poly(acrylamide) (LPA) solution for the application of DNA sequencing. A systematic study is presented of the dependen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a6892f3ea22174d8cb5b77a9ede250b
https://doi.org/10.1021/ac000055j
https://doi.org/10.1021/ac000055j
Autor:
Dan Chisholm, Aram Adourian, Alok K. Srivastava, Norman Tsao, Dieter Schmalzing, Lauren Linton, Paul Matsudaira, Lance Koutny, Paul A. McEwan, Daniel J. Ehrlich
Publikováno v:
Genome Research. 9:853-858
We report results using a microdevice for DNA sequencing using samples from chromosome 17, obtained from the Whitehead Institute Center for Genome Research (WICGR) production line. The device had an effective separation distance of 11.5 cm and a lith
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb85f33ada1263e18fa680fc5f368506
https://doi.org/10.1101/gr.9.9.853
https://doi.org/10.1101/gr.9.9.853
Two-Color Multiplexed Analysis of Eight Short Tandem Repeat Loci with an Electrophoretic Microdevice
Autor:
Daniel J. Ehrlich, Aram Adourian, Lance Koutny, Dan Chisholm, Paul Matsudaira, Dieter Schmalzing
Publikováno v:
Analytical Biochemistry. 270:148-152
Single-channel microfabricated electrophoretic devices equipped with a dual-wavelength laser-induced fluorescence detection system were used for the fast analysis of an eight-loci, two-color multiplex short tandem repeat (STR) system for human identi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b2cae99d68a9e47d2a318e5ee3036a5
https://doi.org/10.1006/abio.1999.4067
https://doi.org/10.1006/abio.1999.4067
Publikováno v:
Journal of Chromatography B: Biomedical Sciences and Applications. 697:175-180
As part of our ongoing effort to develop electrophoretic assay technology for clinical diagnostics, we describe a competitive immunoassay for the determination of serum thyroxine (T4) based on electrophoresis and laser induced fluorescence (LIF). Mea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69adfb6653f7326b6a215267ec84e0d5
https://doi.org/10.1016/s0378-4347(97)00256-9
https://doi.org/10.1016/s0378-4347(97)00256-9
Publikováno v:
Analytical Chemistry. 68:18-22
An immunoassay performed using a microchip electrophoretic system is described. Separation and quantitation of free and bound labeled antigen in a competitive assay are carried out in channels micromachined into fused silica substrates. Such microchi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00b3be4036f877e6e73881f7f05b7393
https://doi.org/10.1021/ac9508311
https://doi.org/10.1021/ac9508311
Autor:
Edward S. Yeung, Lance Koutny
Publikováno v:
Analytical Chemistry. 65:148-152
Normally, data acquisition and processing in chemical measurements are based on equal time (effort) for each data point. This leads to uneven signal-to-noise ratios (S/N) for each point. The authors present an expert system which decides in real time
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f7c77deab1b220e55ec316e4beec2f0
https://doi.org/10.1021/ac00050a010
https://doi.org/10.1021/ac00050a010
Autor:
Lance Koutny, Edward S. Yeung
Publikováno v:
Analytical Chemistry. 65:183-187
Slab-gel electrophoresis is the most common technique for the separation of high molecular weight biomolecules such a proteins. Acrylamide gels, as described by Laemmli, are generally the matrix of choice for the separation of SDS-denatured proteins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::35ce52e56f2053b63a8824f1f1e763ce
https://doi.org/10.1021/ac00050a017
https://doi.org/10.1021/ac00050a017