Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Lan Hsiang Huang"'
Publikováno v:
Analytical Biochemistry. 122:89-93
An enzymatic method has been developed to synthesize 5-methyldeoxycytidine triphosphate (m5dCTP) starting with dCMP and formaldehyde as substrates. Methylation is catalyzed by a dCMP methyltransferase in the presence of tetrahydrofolic acid. The resu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d7089e30a58163845361a3bc87b2484
https://doi.org/10.1016/0003-2697(82)90255-x
https://doi.org/10.1016/0003-2697(82)90255-x
Autor:
Richard Y.-H. Wang, Melanie Ehrlich, Lan-Hsiang Huang, Rana Khan, Xian-Yang Zhang, Youwen Zhou
Publikováno v:
Nucleic Acids Research. 14:9843-9860
Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP indicate that m5CpG is present in the recognition sites
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df6947f888c349bfb461881df9d7998d
https://doi.org/10.1093/nar/14.24.9843
https://doi.org/10.1093/nar/14.24.9843
Publikováno v:
Journal of Biological Chemistry. 264:8811-8814
For the baboon endogenous virus to infect human cells a specific region on chromosome 6 is required for viral DNA replication and integration. In studying the kinetics of baboon endogenous virus DNA replication we show that linear DNA was synthesized
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8c174cda1820d2ba3bdbc5e45ebd0a17
https://doi.org/10.1016/s0021-9258(18)81865-8
https://doi.org/10.1016/s0021-9258(18)81865-8
Publikováno v:
Experimental Cell Research. 179:194-203
Stimulation of β-adrenoreceptors in rat parotid acinar cells in vitro by the β-adrenergic agonist isoproterenol induces steady-state levels of c- fos mRNA and c- fos protein in these cells. A dramatic increase in the steady-state levels of c- fos m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9d408c11cfc120f21c75818ff5396f9
https://doi.org/10.1016/0014-4827(88)90358-8
https://doi.org/10.1016/0014-4827(88)90358-8
Publikováno v:
Nucleic Acids Research. 12:3473-3490
We have partially purified a DNA methyltransferase from human placenta using a novel substrate for a highly sensitive assay of methylation of hemimethylated DNA. This substrate was prepared by extensive nick translation of bacteriophage XP12 DNA, whi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dee3dbf4e2bcddaf0b3dc2a9ef55424
https://doi.org/10.1093/nar/12.8.3473
https://doi.org/10.1093/nar/12.8.3473
Publikováno v:
Nucleic acids research. 10(5)
The ability of thirty Type II restriction endonucleases to cleave five different types of highly modified DNA has been examined. The DNA substrates were derived from relatively large bacteriophage genomes which contain all or most of the cytosine or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e15e9284d7300958901b256ffc92f2c
https://pubmed.ncbi.nlm.nih.gov/6280151
https://pubmed.ncbi.nlm.nih.gov/6280151
Publikováno v:
Biochimica et biophysica acta. 696(1)
Bacteriophage XP-12-infected Xanthomonas oryzae have been found to be a source of a kinase preparation which converts m5dCMP to m5dCDP and then to m5dCTP using ATP as the phosphate donor. Optimal formation of the triphosphate required the presence of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0746995221bf2e20a642a2a8f401e9d1
https://pubmed.ncbi.nlm.nih.gov/7082669
https://pubmed.ncbi.nlm.nih.gov/7082669
Publikováno v:
Biochimica et biophysica acta. 741(2)
After digestion by TaqI or nicking by DNAase I, five highly modified bacteriophage DNAs were tested as substrates for T4 DNA ligase. The DNAs used were from phages T4, XP12, PBS1, SP82, and SP15, which contain as a major base either glucosylated 5-hy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b79dfede0ab6a6fd40afe06845627b27
https://pubmed.ncbi.nlm.nih.gov/6652091
https://pubmed.ncbi.nlm.nih.gov/6652091
Autor:
Miguel A. Gama-Sosa, Rose Marie Midgett, Lan Hsiang Huang, Kenneth C. Kuo, Melanie Ehrlich, Roy A. McCune, Charles W. Gehrke
Analysis of the total base composition of DNA from seven different normal human tissues and eight different types of homogeneous human cell populations revealed considerable tissue-specific and cell-specific differences in the extent of methylation o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47cd3634d4d3378b622ffe8b4b5bcd5e
https://europepmc.org/articles/PMC320645/
https://europepmc.org/articles/PMC320645/
Publikováno v:
Nature. 308(5956)
The methylation of vertebrate DNA at the 5-position of approximately 3-10% of its cytosine residues occurs in a sequence-specific and tissue-specific manner and has been implicated in the control of transcription. How these differences are establishe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8ba1a24b3b137e2812348684289c6ff
https://pubmed.ncbi.nlm.nih.gov/6538260
https://pubmed.ncbi.nlm.nih.gov/6538260