De novo gene synthesis by an antiviral reverse transcriptase.

Autor: Tang S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Conte V; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Zhang DJ; Department of Biological Sciences, Columbia University, New York, NY, USA., Žedaveinytė R; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Wiegand T; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Tang LC; Department of Biological Sciences, Columbia University, New York, NY, USA., Wang M; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Walker MWG; Department of Biological Sciences, Columbia University, New York, NY, USA., George JT; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Berchowitz LE; Department of Genetics and Development, Columbia University, New York, NY, USA.; Taub Institute for Research on Alzheimer's and the Aging Brain, New York, NY, USA., Jovanovic M; Department of Biological Sciences, Columbia University, New York, NY, USA., Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2024 Oct 04; Vol. 386 (6717), pp. eadq0876. Date of Electronic Publication: 2024 Oct 04.

Antagonistic conflict between transposon-encoded introns and guide RNAs.

Autor: Žedaveinytė R; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Meers C; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Le HC; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Mortman EE; Department of Genetics and Development, Columbia University, New York, NY 10032, USA., Tang S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Pesari SR; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Gelsinger DR; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Wiegand T; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA., Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.

Publikováno v: Science (New York, N.Y.) [Science] 2024 Jul 12; Vol. 385 (6705), pp. eadm8189. Date of Electronic Publication: 2024 Jul 12.

Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases.

Autor: Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., King RT; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Halpin-Healy TS; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.; Regeneron Pharmaceuticals, Inc., Tarrytown, NY, USA., Klompe SE; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.; Department of Genomes and Genetics, Institut Pasteur, Paris, France., Hogan MI; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.; Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA, USA., Vo PLH; Department of Molecular Pharmacology and Therapeutics, Columbia University, New York, NY, USA.; Vertex Pharmaceuticals, Inc., Boston, MA, USA., Tang S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Chavez A; Department of Pathology and Cell Biology, Columbia University, New York, NY, USA.; Department of Pediatrics, University of California, San Diego, La Jolla, CA, USA., Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. shsternberg@gmail.com.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2024 Jan; Vol. 42 (1), pp. 87-98. Date of Electronic Publication: 2023 Mar 29.

Novel recombinases for large DNA insertions.

Autor: Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA., Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. shsternberg@gmail.com.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2023 Apr; Vol. 41 (4), pp. 471-472.