Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

Autor: Lamiae Abdeladim, Katherine S. Matho, Solène Clavreul, Pierre Mahou, Jean-Marc Sintes, Xavier Solinas, Ignacio Arganda-Carreras, Stephen G. Turney, Jeff W. Lichtman, Anatole Chessel, Alexis-Pierre Bemelmans, Karine Loulier, Willy Supatto, Jean Livet, Emmanuel Beaurepaire

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)

Multicolour images are difficult to acquire with large-scale microscopy approaches. Here the authors present a microtome-assisted microscope capable of trichromatic two-photon excitation and label-free nonlinear modalities based on wavelength mixing,

Externí odkaz: https://doaj.org/article/936f22a7c3dd47068d7ddbe4fade0e58

Publisher Correction: Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy

Autor: Lamiae Abdeladim, Katherine S. Matho, Solène Clavreul, Pierre Mahou, Jean-Marc Sintes, Xavier Solinas, Ignacio Arganda-Carreras, Stephen G. Turney, Jeff W. Lichtman, Anatole Chessel, Alexis-Pierre Bemelmans, Karine Loulier, Willy Supatto, Jean Livet, Emmanuel Beaurepaire

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-1 (2019)

Affiliation 4 incorrectly read ‘University of the Basque Country (Ikerbasque), University of the Basque Country and Donostia International Physics Center, San Sebastian 20018, Spain.’ Also, the affiliations of Ignacio Arganda-Carreras with ‘IKE

Externí odkaz: https://doaj.org/article/14c92ea4f09f4501a3fc3bf6cc95a03b

Fast cardiac imaging in live embryos using multiphoton light-sheet microscopy at low laser repetition rate

Autor: Vincent Maioli, Antoine Boniface, Pierre Mahou, Júlia Ferrer Ortas, Lamiae Abdeladim, Emmanuel Beaurepaire, Willy Supatto

Publikováno v: Advances in Microscopic Imaging III.

We propose a framework to quantify photodamage in multiphoton light-sheet microscopy. Using cardiac imaging in live zebrafish embryos, we demonstrate an order of magnitude signal enhancement is safely obtained by adjusting the laser repetition rate.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a95a35a6f300cade62eb6fb7b5b89a8
https://doi.org/10.1117/12.2615701

Cortical astrocytes develop in a plastic manner at both clonal and cellular levels

Autor: Jason Durand, Sio-Hoi Ieng, Edwin Hernández-Garzón, Emmanuel Beaurepaire, Solène Clavreul, Gilles Bonvento, Lamiae Abdeladim, Raphaëlle Barry, Dragos Niculescu, Jean Livet, Karine Loulier

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Nature Communications
Nature Communications, 2019, 10 (1), ⟨10.1038/s41467-019-12791-5⟩
Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-12791-5⟩

Astrocytes play essential roles in the neural tissue where they form a continuous network, while displaying important local heterogeneity. Here, we performed multiclonal lineage tracing using combinatorial genetic markers together with a new large vo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33cd52955e66411ee84f47c362a0f5c6
http://link.springer.com/article/10.1038/s41467-019-12791-5

Fast in vivo multiphoton microscopy using optimized light-sheet illumination

Autor: Pierre Mahou, Antoine Boniface, Emmanuel Beaurepaire, Lamiae Abdeladim, Vincent Maioli, Júlia Ferrer Ortas, Willy Supatto

Publikováno v: Multiphoton Microscopy in the Biomedical Sciences XXI
SPIE BiOS
SPIE BiOS, Mar 2021, Online Only, United States. ⟨10.1117/12.2578703⟩

International audience; Light-sheet fluorescence microscopy is a method of choice for multiscale live imaging. Indeed, its orthogonal geometry results in high acquisition speed, large field-of-view and low photodamage. Its combination with multiphoto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecc9111ecf3e8185b453bc646fc7b0cf
https://hal.science/hal-03427304/document