Macromolecular condensation organizes nucleolar sub-phases to set up a pH gradient.

Autor: King MR; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Ruff KM; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Lin AZ; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Pant A; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Farag M; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Lalmansingh JM; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Wu T; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Department of Electrical and Systems Engineering, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Fossat MJ; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Ouyang W; Department of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, CA, USA; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden., Lew MD; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Department of Electrical and Systems Engineering, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Lundberg E; Department of Bioengineering, Schools of Engineering and Medicine, Stanford University, Stanford, CA, USA; Department of Pathology, School of Medicine, Stanford University, Stanford, CA, USA; Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH-Royal Institute of Technology, Stockholm, Sweden., Vahey MD; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA., Pappu RV; Department of Biomedical Engineering, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA; Center for Biomolecular Condensates, James McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO, USA. Electronic address: pappu@wustl.edu.

Publikováno v: Cell [Cell] 2024 Apr 11; Vol. 187 (8), pp. 1889-1906.e24. Date of Electronic Publication: 2024 Mar 18.

SOURSOP: A Python Package for the Analysis of Simulations of Intrinsically Disordered Proteins.

Autor: Lalmansingh JM; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.; Center for Biomolecular Condensates, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Keeley AT; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.; Department of Chemistry, University of Illinois Urbana-Champaign, Urbana-Champaign, Illinois 61801, United States., Ruff KM; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.; Center for Biomolecular Condensates, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Pappu RV; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, United States.; Center for Biomolecular Condensates, Washington University in St. Louis, St. Louis, Missouri 63130, United States., Holehouse AS; Center for Biomolecular Condensates, Washington University in St. Louis, St. Louis, Missouri 63130, United States.; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110, United States.

Publikováno v: Journal of chemical theory and computation [J Chem Theory Comput] 2023 Aug 22; Vol. 19 (16), pp. 5609-5620. Date of Electronic Publication: 2023 Jul 18.

Dynamical control enables the formation of demixed biomolecular condensates.

Autor: Lin AZ; Division of Biology and Biomedical Sciences, Plant and Microbial Biosciences, Washington University in St. Louis, St. Louis, MO 63130, USA., Ruff KM; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Jalihal A; Department of Biology, University of North Carolina, Chapel Hill, NC., Dar F; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., King MR; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Lalmansingh JM; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Posey AE; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA., Seim I; Department of Biology, University of North Carolina, Chapel Hill, NC., Gladfelter AS; Department of Biology, University of North Carolina, Chapel Hill, NC., Pappu RV; Department of Biomedical Engineering and Center for Biomolecular Condensates, James F. McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.

Publikováno v: Research square [Res Sq] 2023 Feb 08. Date of Electronic Publication: 2023 Feb 08.

Uncovering Non-random Binary Patterns Within Sequences of Intrinsically Disordered Proteins.

Autor: Cohan MC; Department of Biomedical Engineering and Center for Science & Engineering of Living Systems (CSELS), Washington University in St. Louis, MO 63130, USA., Shinn MK; Department of Biomedical Engineering and Center for Science & Engineering of Living Systems (CSELS), Washington University in St. Louis, MO 63130, USA., Lalmansingh JM; Department of Physics, Washington University in St. Louis, MO 63130, USA., Pappu RV; Department of Biomedical Engineering and Center for Science & Engineering of Living Systems (CSELS), Washington University in St. Louis, MO 63130, USA. Electronic address: pappu@wustl.edu.

Publikováno v: Journal of molecular biology [J Mol Biol] 2022 Jan 30; Vol. 434 (2), pp. 167373. Date of Electronic Publication: 2021 Dec 01.