Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Lalio Djondjurov"'
Publikováno v:
Cancer Letters. 106:51-58
A two-step procedure for isolation of chromatin fragments released from apoptotic cells is described. The first step includes extraction of the chromatin fragments by a hypotonic treatment of isolated nuclei. The second step provides stabilization of
Publikováno v:
European Journal of Biochemistry. 207:887-894
This investigation is centered on the intranuclear localization of transcribed and nontranscribed c-myc sequences in human Namalva-S cells bearing t(8;14) translocation. Southern hybridization showed that the breakpoint in the truncated allele of c-m
Publikováno v:
Journal of Cell Science. 95:667-674
In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates
Autor:
Rossen M. Donev, Lalio Djondjurov
Publikováno v:
DNA and cell biology. 18(2)
Using electron microscopy (EM), we have examined three structural domains of the mitotic chromosome scaffold of mouse erythroleukemia (MEL) Friend cells with different morphologic organization: centromeric, intermediate, and telomeric. The intermedia
Publikováno v:
DNA and cell biology. 15(4)
This study characterizes the fragmented DNA of mouse erythroleukemia (MEL) cells spontaneously entering apoptosis. Fragmented DNA was isolated by introducing a novel procedure that ensured a complete extraction of the characteristic oligonucleosomal
Publikováno v:
Plant cell reports. 11(7)
In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and
Publikováno v:
Experimental cell research. 193(2)
By using various approaches, we have observed in cycling mouse erythroleukemia cells a characteristic sequential deposition of newly synthesized histones. Control experiments confirmed that this deposition is replicative and is not associated with a
Autor:
Lalio Djondjurov, Howard Holtzer
Publikováno v:
Analytical Biochemistry. 94:274-277
A procedure is described for recovery of proteins after separation in analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A high percentage of recovery is achieved (60–80%) for the range from 5–50 μg protein loaded, allowing th
Publikováno v:
Biochemical Journal. 264:785-791
This study examines the histones pools in the nucleosol and cytosol of proliferating Friend cells. By using the conventional approach, detectable amounts of these molecules were found in both compartments; however, only H3 and H2B were identified in
Publikováno v:
Experimental Cell Research. 180:94-105
This study examines the turnover of the core histones in proliferating Friend cells. It was calculated that these proteins turn over with half-lives of 21.6 days for H2A, 13.8 days for H2B, 43.3 days for H3, and 138.6 days for H4. The significant dif