Evidence that conserved residues Cys-62 and Cys-154 within the Azotobacter vinelandii nitrogenase MoFe protein α-subunit are essential for nitrogenase activity but conserved residues His-83 and Cys-88 are not.

Autor: Dean DR; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA., Setterquist RA; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA., Brigle KE; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA., Scott DJ; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA., Laird NF; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA., Newton WE; Department of Anaerobic Microbiology, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USADepartment of Biochemistry, Virgina Polytechnic Institute and State University. Blacksburg, Virgina 24061, USAThe Western Regional Research Center, USDA-ARS, Albarv, California 94710, USA.

Publikováno v: Molecular microbiology [Mol Microbiol] 1990 Sep; Vol. 4 (9), pp. 1505-1512.

Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii.

Autor: Jacobson MR; Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061., Cash VL, Weiss MC, Laird NF, Newton WE, Dean DR

Publikováno v: Molecular & general genetics : MGG [Mol Gen Genet] 1989 Oct; Vol. 219 (1-2), pp. 49-57.