TEM ExosomeAnalyzer: a computer-assisted software tool for quantitative evaluation of extracellular vesicles in transmission electron microscopy images

Autor: Anna Kotrbová, Karel Štěpka, Martin Maška, Jakub Jozef Pálenik, Ladislav Ilkovics, Dobromila Klemová, Marek Kravec, František Hubatka, Zankruti Dave, Aleš Hampl, Vítězslav Bryja, Pavel Matula, Vendula Pospíchalová

Publikováno v: Journal of Extracellular Vesicles, Vol 8, Iss 1 (2019)

Extracellular vesicles (EVs) function as important conveyers of information between cells and thus can be exploited as drug delivery systems or disease biomarkers. Transmission electron microscopy (TEM) remains the gold standard method for visualisat

Externí odkaz: https://doaj.org/article/e8422357174045708c3588dd84e57d8e

Detergent-modified catalytic and enzymomimetic activity of silver and palladium nanoparticles biotemplated by Pyrococcus furiosus ferritin

Autor: Marek Remes, Simona Dostalova, Zbynek Heger, Esther M. Sánchez-Carnerero, Vladimir Pekarik, Marie Peskova, Ladislav Ilkovics, David Hynek

Publikováno v: Journal of Colloid and Interface Science. 537:20-27

Palladium and silver nanoparticles (NPs) anchored at the outer surface of ferritin form stable suspension of non-coated particles that possess several catalytic and enzymomimetic activities. These activities are strongly affected by detergents that s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b020037d69292aabb414048ebaff64dd
https://doi.org/10.1016/j.jcis.2018.11.005

Microscopic monitoring provides information on structure and properties during biocatalyst immobilization

Autor: Šárka Bidmanová, Eva Hrdlickova, Zbynek Prokop, Josef Jaroš, Aleš Hampl, Jiri Damborsky, Ladislav Ilkovics

Publikováno v: Biotechnology Journal. 9:852-860

Enzymes have a wide range of applications in different industries owing to their high specificity and efficiency. Immobilization is often used to improve biocatalyst properties, operational stability, and reusability. However, changes in the structur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4f05c593ffacfcbbf38e76031495042
https://doi.org/10.1002/biot.201300049

Ultrastructural characteristics and variability of vegetative reproduction inFellomyces penicillatus

Autor: Vladimíra Ramíková, Dobromila Klemová, Marie Kopecká, Ladislav Ilkovics, Wladyslav Golubev

Publikováno v: Journal of Basic Microbiology. 52:531-538

The yeast strains VKM Y-2977 and VKM Y-2978, derived from the isolate Pa-202, were examined for their physiological properties and mycocin sensitivities and studied by light, phase-contrast, fluorescence, transmission and scanning electron microscopy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::934d3c75c78e34ca82a6d70852ad8c42
https://doi.org/10.1002/jobm.201100405

Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer

Autor: Karol Kaiser, Igor Crha, Vít Weinberger, Anna Kotrbova, Vitezslav Bryja, Dobromila Klemová, Ladislav Ilkovics, Jan Svoboda, Vendula Pospichalova, Aleš Hampl, Zankruti Dave, Lubos Minar, Eva Jandáková

Publikováno v: Journal of Extracellular Vesicles
Journal of Extracellular Vesicles; Vol 4 (2015) incl supplements
Journal of Extracellular Vesicles, Vol 4, Iss 0, Pp 1-15 (2015)

Flow cytometry is a powerful method, which is widely used for high-throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several chall

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29115d99d7154756205e76abcf791bcc
https://pubmed.ncbi.nlm.nih.gov/25833224