Quantitative parameters of bacterial RNA polymerase open-complex formation, stabilization and disruption on a consensus promoter

Autor: Mona Seifert, Anssi M. Malinen, Eugeniu Ostrofet, Monika Spermann, Flavia S. Papini, Martin Depken, Jelmer Cnossen, Pim P. B. America, Subhas C. Bera, David Dulin, Santeri Maatsola

Publikováno v: Bera, S C, America, P P B, Maatsola, S, Seifert, M, Ostrofet, E, Cnossen, J, Spermann, M, Papini, F S, Depken, M, Malinen, A M & Dulin, D 2022, ' Quantitative parameters of bacterial RNA polymerase open-complex formation, stabilization and disruption on a consensus promoter ', Nucleic acids research, vol. 50, no. 13, pp. 7511-7528 . https://doi.org/10.1093/nar/gkac560
Nucleic acids research, 50(13), 7511-7528. Oxford University Press
Nucleic acids research, 50(13)

Transcription initiation is the first step in gene expression, and is therefore strongly regulated in all domains of life. The RNA polymerase (RNAP) first associates with the initiation factorσto form a holoenzyme, which binds, bends and opens the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45f6e38ca3ee2e0673f2e56fa20fb2a4
https://research.vu.nl/en/publications/11e1a01f-cd32-446b-ae41-073da4bd11f0

Regulating the T7 RNA Polymerase Expression in E. coli BL21 (DE3) to Provide More Host Options for Recombinant Protein Production

Autor: Ying-Shuang Xu, Yu-Zhou Wang, Yun-Qi Liu, Zi-Jia Li, Zi-Xu Zhang, Fei Du, Xiao-Man Sun

Publikováno v: Microbial Cell Factories, Vol 20, Iss 1, Pp 1-10 (2021)
Microbial Cell Factories

Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (PlacUV5),

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::455da28635f3eff595e654b232692ea2
https://doi.org/10.21203/rs.3.rs-678077/v1

CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

Autor: Shiyan Qiao, Changchuan Ye, Mengjie Yang, Xi Chen, Xiangfang Zeng

Publikováno v: Journal of Biological Engineering, Vol 15, Iss 1, Pp 1-16 (2021)
Journal of Biological Engineering

T7 Expression System is a common method of ensuring tight control and high-level induced expression. However, this system can only work in some bacterial strains in which the T7 RNA Polymerase gene resides in the chromosome. In this study, we success

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5783ebeab364c28bf29c286568416dfd
https://doi.org/10.21203/rs.3.rs-339217/v1

Downregulation of T7 RNA polymerase transcription enhances pET-based recombinant protein production via suppression of Escherichia coli BL21 (DE3) programmed cell death

Autor: Yu Jiang, Yang Junjie, Xiao-Man Sun, Liang Yan, Ling-Ru Wang, Rongsheng Tao, Sheng Yang, Hai-Feng Yang, Jing-Gang Wang, Zi-Xu Zhang

E. coli BL21 (DE3) is an excellent and widely used host for recombinant protein production. Many variant hosts were developed from from BL21 (DE3), but improving the expression of specific proteins remains a major challenge in biotechnology. In this

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0a2ba19597b98a4eea065d0411e44b19
https://doi.org/10.22541/au.159301729.96050838

Biotechnological approach for the production of L-asparaginase from locally Bacillus subtilis isolate

Autor: Wafaa K. Hegazy, Mohamed S. Abdel-Salam, Maysa E. Moharam

Publikováno v: Egyptian Pharmaceutical Journal. 19:155

Background and objectives L-asparaginase is a therapeutic enzyme used for the treatment of hematopoietic diseases, for example, acute lymphoblastic leukemia. It has many applications other than as an anticancer agent, which includes in the treatment

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::28925eef6a9320c145bb2ae081f6c83c
https://doi.org/10.4103/epj.epj_35_19