Efficient and scalable clarification of Orf virus from HEK suspension for vaccine development.

Autor: Pagallies F; Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany., Labisch JJ; Lab Essentials Applications Development, Sartorius, Otto-Brenner-Straße 20, 37079 Göttingen, Germany., Wronska M; Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany.; PRiME Vector Technologies, Herrenberger Straße 24, 72070 Tübingen, Germany., Pflanz K; Lab Essentials Applications Development, Sartorius, Otto-Brenner-Straße 20, 37079 Göttingen, Germany., Amann R; Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany.; PRiME Vector Technologies, Herrenberger Straße 24, 72070 Tübingen, Germany.

Publikováno v: Vaccine: X [Vaccine X] 2024 Mar 13; Vol. 18, pp. 100474. Date of Electronic Publication: 2024 Mar 13 (Print Publication: 2024).

Stability studies for the identification of critical process parameters for a pharmaceutical production of the Orf virus.

Autor: Eilts F; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr.14, 35390 Giessen, Germany., Labisch JJ; Lab Essentials Applications Development, Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Goettingen, Germany; Institute of Technical Chemistry, Leibniz University Hannover, Callinstraße 3-9, 30167 Hannover, Lower Saxony, Germany., Orbay S; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr.14, 35390 Giessen, Germany., Harsy YMJ; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr.14, 35390 Giessen, Germany., Steger M; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr.14, 35390 Giessen, Germany., Pagallies F; Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15/3.008, 72076 Tuebingen, Germany., Amann R; Department of Immunology, University of Tuebingen, Auf der Morgenstelle 15/3.008, 72076 Tuebingen, Germany; Prime Vector Technologies, Herrenberger Straße 24, 72070 Tuebingen, Germany., Pflanz K; Lab Essentials Applications Development, Sartorius Stedim Biotech GmbH, August-Spindler-Straße 11, 37079 Goettingen, Germany., Wolff MW; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr.14, 35390 Giessen, Germany. Electronic address: michael.wolff@lse.thm.de.

Publikováno v: Vaccine [Vaccine] 2023 Jul 19; Vol. 41 (32), pp. 4731-4742. Date of Electronic Publication: 2023 Jun 21.

Steric exclusion chromatography of lentiviral vectors using hydrophilic cellulose membranes.

Autor: Labisch JJ; Lab Essentials Applications Development, Sartorius Stedim Biotech GmbH, Göttingen, Lower Saxony, Germany; Institute of Technical Chemistry, Leibniz University Hannover, Hanover, Lower Saxony, Germany. Electronic address: jennifer.labisch@sartorius.com., Kassar M; Lab Essentials Applications Development, Sartorius Stedim Biotech GmbH, Göttingen, Lower Saxony, Germany; Karlsruhe Institute of Technology, Institute of Process Engineering and Life Sciences, Biomolecular Separation Engineering, Karlsruhe, Baden-Württemberg, Germany., Bollmann F; Marketing Separation Technologies, Sartorius Stedim Biotech GmbH, Göttingen, Lower Saxony, Germany., Valentic A; Karlsruhe Institute of Technology, Institute of Process Engineering and Life Sciences, Biomolecular Separation Engineering, Karlsruhe, Baden-Württemberg, Germany., Hubbuch J; Karlsruhe Institute of Technology, Institute of Process Engineering and Life Sciences, Biomolecular Separation Engineering, Karlsruhe, Baden-Württemberg, Germany., Pflanz K; Lab Essentials Applications Development, Sartorius Stedim Biotech GmbH, Göttingen, Lower Saxony, Germany.

Publikováno v: Journal of chromatography. A [J Chromatogr A] 2022 Jul 05; Vol. 1674, pp. 463148. Date of Electronic Publication: 2022 May 14.

A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling.

Autor: Labisch JJ; Institute of Technical Chemistry, Leibniz University Hannover, Callinstr. 5, 30167, Hannover, Germany; Research & Development, Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany. Electronic address: jennifer.labisch@sartorius.com., Bollmann F; Research & Development, Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany., Wolff MW; Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen (THM), Wiesenstr. 14, 35390, Giessen, Germany., Pflanz K; Research & Development, Sartorius Stedim Biotech GmbH, August-Spindler-Str. 11, 37079, Goettingen, Germany.

Publikováno v: Journal of biotechnology [J Biotechnol] 2021 Jan 20; Vol. 326, pp. 11-20. Date of Electronic Publication: 2020 Dec 07.