Assessing microbial diversity in Yellowstone National Park hot springs using a field deployable automated nucleic acid extraction system

Autor: Jason M. Wood, Camilla Urbaniak, Ceth Parker, Nitin Kumar Singh, Season Wong, Arunkumar Arumugam, Dana J. Skorupa, Ashlyn Hemmah, Phoebe Laaguiby, Fathi Karouia, Brent M. Peyton, Scott Tighe, Kasthuri Venkateswaran

Publikováno v: Frontiers in Ecology and Evolution, Vol 12 (2024)

Microbial diversity estimation involves extracting nucleic acids from intricate sample matrices. Preparing nucleic acid samples is time-consuming, necessitating effective cell lysis and obtaining pure, inhibitor-free nucleic acid purifications before

Externí odkaz: https://doaj.org/article/f595ff73c82a4193b406034b869efa83

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step.

Autor: Emily A Bruce, Meei-Li Huang, Garrett A Perchetti, Scott Tighe, Pheobe Laaguiby, Jessica J Hoffman, Diana L Gerrard, Arun K Nalla, Yulun Wei, Alexander L Greninger, Sean A Diehl, David J Shirley, Debra G B Leonard, Christopher D Huston, Beth D Kirkpatrick, Julie A Dragon, Jessica W Crothers, Keith R Jerome, Jason W Botten

Publikováno v: PLoS Biology, Vol 18, Iss 10, p e3000896 (2020)

The ongoing COVID-19 pandemic has created an unprecedented need for rapid diagnostic testing. The World Health Organization (WHO) recommends a standard assay that includes an RNA extraction step from a nasopharyngeal (NP) swab followed by reverse tra

Externí odkaz: https://doaj.org/article/54f6f0859c2e48d5a294bcb9407b675d

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DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP.

Autor: Bruce EA; Department of Medicine, Division of Immunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Huang ML; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Perchetti GA; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Tighe S; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Laaguiby P; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Hoffman JJ; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Gerrard DL; Department of Pathology and Laboratory Medicine, University of Vermont Medical Center, Burlington VT, 05401, USA., Nalla AK; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Wei Y; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA., Greninger AL; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA., Diehl SA; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA., Shirley DJ; IXIS LLC, Data Science Division, Burlington, VT 05401, USA., Leonard DGB; Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA., Huston CD; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA., Kirkpatrick BD; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA.; Department of Medicine, Division of Infectious Disease, University of Vermont Medical Center, Burlington VT, 05401, USA., Dragon JA; Vermont Integrative Genomics Resource, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA., Crothers JW; Department of Pathology and Laboratory Medicine, Robert Larner, M.D. College of Medicine, University of Vermont and the University of Vermont Health Network, Burlington VT, 05405, USA., Jerome KR; Virology Division, Department of Laboratory Medicine, University of Washington, Seattle WA 98195, USA.; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle WA 98109, USA., Botten JW; Department of Medicine, Division of Immunobiology, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington VT, 05405, USA.; Vaccine Testing Center, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT, 05405 USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2020 Apr 06. Date of Electronic Publication: 2020 Apr 06.